温经异位康冲剂治疗子宫内膜异位症的实验研究

目的观察温经异位康冲剂治疗子宫内膜异位症及痛经的作用。方法手术制备大鼠子宫内膜异位模型，观察形态学改变，配合子宫内膜抗体及雌二醇、孕酮、ＰＧＦ２α水平检测；以及动物子宫活动、抗炎等试验。结果本品能抑制大鼠子宫内膜异位模型，降低子宫内膜抗体及阳性率、血清雌二醇、孕酮及ＰＧＦ２α（Ｐ＜０．０１）。病理学观察，异位子宫的内膜腺体及腺上皮明显萎缩，呈剂量依赖性；其作用与丹那唑相似，可抑制大鼠离体子宫和家兔在体子宫的收缩活动，降低子宫的收缩幅度、频率和张力，并能拮抗缩宫素和麦角新碱对离体及在体子宫的兴奋作用，其作用比月月舒和丹那唑强。对大鼠卵巢的生长及卵泡发育无明显影响。对蛋白诱发的大鼠足肿胀及二甲苯所致的小鼠耳肿胀有良好的抑制作用。对醋酸致小鼠扭体反应和正常大鼠的免疫功能无明显影响。

Experimental Study on AntiEndometriosis of wenjing Yiweikang Granule

Objective: To investigate the therapeutic effect of Wenjingyiweikang keli on endometriosis and dysmenorrhea. METHODS: The rat endometriosis model was produced by surgery,we observed the change of uterus tissue morphology,the antibody level of antiendometrium and the serum level of estradiol,progesterone and PGF2. The effect on uterus activation,inflammation and pain was also observed. RESULTS: Wenjingyiweikang keli could inhibit the endometriosis of rat,reduced the antibody level of antiendometrium,decreaser the level of estradiol,progesterone and PGF2 n serum (p<0.01).Through pathological observation,the glands and glandular epithelium of abnormal endometrium were obviously withered,the effect was dose dependent,similar to Danazol.The uterus constriction of rat in vitro and of rbbit in vivo was both inhibited.In vitro,the range,the frequency and the tension of the constriction were decreased,the effectwas dose dependent,while in vivo,only the tension of the constriction was inhibited.So did the uterus constriction produce by oxytocin and ergotocine,and the effect was stronger than the effect of Yueyueshu and Danazol.The growth of ovary and folliculus were not effected.The eggwhiteinduced paw swelling of rat and the xyleneinduced auricular swelling of mouse were inhibited.The mouse twisting induced by acetic acid was not influenced,either.The normal immune system of rat was not influenced.