| 蛋白质电泳考马斯亮蓝G250染色方法改良 |
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| 引用本文： | 汪静,袁琳,陈晓明.蛋白质电泳考马斯亮蓝G250染色方法改良[J].医学分子生物学杂志,2006,3(6):423-425. |
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| 作者姓名： | 汪静 袁琳 陈晓明 |
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| 作者单位： | 1. 武汉理工大学生物材料与工程研究中心,武汉市,430070;武汉市江岸区妇幼保健院,武汉市,430017
2. 武汉理工大学生物材料与工程研究中心,武汉市,430070 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的建立一种快速,简易的SDS-PAGE染色方法。方法用考马斯亮蓝G250和磷酸作为染色液,用水作为脱色液。并和考马斯亮蓝R250染色方法比较。结果该方法染色背景浅,脱色简便,且灵敏度和考马斯亮蓝R250染色方法相当。结论改进的考马斯亮蓝G250染色方法更加简便、经济、快速。适合于快速分析。
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| 关 键 词： | 电泳 染色方法 考马斯亮蓝G250 |
| 修稿时间： | 2006年6月19日 |
An Improved Technique for Protein Staining with CBB G250 |
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| WANG Jing,YUAN Lin,CHEN Xiaoming.An Improved Technique for Protein Staining with CBB G250[J].Journal of Medical Molecular Biology,2006,3(6):423-425. |
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| Authors: | WANG Jing YUAN Lin CHEN Xiaoming |
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| Abstract: | Objective To establish a quick and easy method for SDS-PAGE protein staining.Method The Coomassie Brilliant Blue G250 was dissolved into the ethanol and phosphate,then the SDS-PAGE was stained with the solution.Several hours after staining of the protein,the SDS-PAGE was washed with H_ 2O.The results were compared with traditional method(Coomassie Brilliant Blue R250).Result The gelatin stained with this solution had a clear background and destaining was easy and equally sensitive as compared with traditional method.Conclusion The modified method was simple,fast,economical and suitable for quick-testing. |
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| Keywords: | electrophoresis staining method Coomassie Brilliant Blue G250 |
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