| 木犀草素与牛血清白蛋白相互作用的荧光光谱法研究 |
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| 引用本文： | 蔡静,杜迎翔,汪豪,谢玉荣,罗天雪,丁可. 木犀草素与牛血清白蛋白相互作用的荧光光谱法研究[J]. 海峡药学, 2010, 22(6): 42-45 |
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| 作者姓名： | 蔡静 杜迎翔 汪豪 谢玉荣 罗天雪 丁可 |
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| 作者单位： | 中国药科大学分析化学教研室,南京,210009;中国药科大学分析化学教研室,南京,210009;中国药科大学药物质量与安全预警教育部重点实验室,南京,210009;中国药科大学现代中药教育部重点实验室,南京,210009;中国药科大学天然药物化学教研室,南京,210009 |
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| 摘 要： | 本文首次采用荧光光谱法研究传统中药白毛夏枯草中活性成分木犀草素与牛血清白蛋白（BSA）的猝灭机制。实验研究了木犀草素在291 k和310 k条件下与BSA的猝灭情况,通过计算求得不同温度下的热力学参数。结果表明木犀草素对牛血清白蛋白荧光的猝灭机制属于静态猝灭过程,二者之间的作用力主要为范德华力和氢键。此外,实验还考察了木犀草素对BSA构象的影响。
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| 关 键 词： | 木犀草素 牛血清白蛋白 白毛夏枯草 荧光光谱法 药物相互作用 |
Study on the interaction between luteolin and bovine serum albumin by fluorescence spectroscopy |
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| CAI Jing,DU Ying-xiang,WANG Hao,XIE Yu-rong,LUO Tian-xue,DING Ke. Study on the interaction between luteolin and bovine serum albumin by fluorescence spectroscopy[J]. Strait Pharmaceutical Journal, 2010, 22(6): 42-45 |
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| Authors: | CAI Jing DU Ying-xiang WANG Hao XIE Yu-rong LUO Tian-xue DING Ke |
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| Affiliation: | 1.Department of Analytical Chemistry,China Pharmaceutical University,Nanjing 210009,China;2.Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education),China Pharmaceutical University,Nanjing 210009,China;3.Key Laboratory of Modern Chinese Medicines(Ministry of Education),China Pharmaceutical University,Nanjing 210009,China;4.Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China) |
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| Abstract: | The interaction between luteolin,which was the active constituent of the traditional Chinese medicine Decumbent bugle herb,with bovine serum albumin（BSA） had been studied by fluorescence spectrometry for the first time.In this paper,the interaction between luteolin with BSA were investigated at 291K and 310K by fluorescence spectroscopy,respectively.The thermodynamic parameters under different temperatures were calculated.The data obtained from fluorescence quenching experiments showed that luteolin was bound to BSA and the quenching type was static quenching.The results indicated that the interactions between luteolin with BSA were driven mainly van der Waals forces and hydrogen bonds.Besides,the interaction of BSA with luteolin in the conformation of BSA was also investigated. |
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| Keywords: | Luteolin Bovine serum albumin Decumbent bugle herb Fluorescence spectrometry Drug interactions |
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