| 大鼠神经干细胞向少突胶质细胞定向分化的观察 |
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| 引用本文： | 余沪荣,汪洋,沈馨亚.大鼠神经干细胞向少突胶质细胞定向分化的观察[J].解剖学杂志,2005,28(5):526-528,F0002. |
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| 作者姓名： | 余沪荣 汪洋 沈馨亚 |
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| 作者单位： | 复旦大学上海医学院人体解剖学与组织胚胎学系,上海200032 |
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| 摘 要： | 目的：研究神经干细胞（NSCs）向少突胶质细胞（OLs）的定向分化。方法：以大鼠14d胚胎海马分离得到的NSCs为模型，利用免疫组化、图像分析等方法观察了存bFGF存在下，不同浓度的PDGF-AA对NSCs分化为OLs的作用以及胎牛血清对NSCs分化的影响。结果：加入PDGFAA使NSCs更多地分化为OLs,且不同浓度的PDGF-AA对OLS分化的影响不同。结论：联合应用PDGF-AA和bFGF,可促进NSCs定向分化为OLs；在bFGF存在下，以PDGF-AA 40ng／ml＋0．5％FBS为最佳浓度，可促进NSCs向OLs分化、成熟。
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| 关 键 词： | 神经干细胞 少突胶质细胞 血小板洐生生长因子 细胞分化 |
Observation on differentiation of neural stem cells into oligodendrocytes in rats |
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| YU Hu-Rong,WANG Yang,SHEN Xin-Ya.Observation on differentiation of neural stem cells into oligodendrocytes in rats[J].Chinese Journal of Anatomy,2005,28(5):526-528,F0002. |
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| Authors: | YU Hu-Rong WANG Yang SHEN Xin-Ya |
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| Abstract: | Objective: To study the differentiation of neural stem cells(NSCs) into oligodendrocytes(OLs).Methods: The model of NSCs were established with the hippocampus of embryonic(E14) rats.NSCs were treated with PDGF conditioned medium combined with bFGF. Cultured NSCs were observed during their differentiation into oligodendrocytes.The effects of different concentrations of PDGF-AA and the effect of FBS were studied by immunohistochemistry and image analysis.Results: The PDGF-AA promoted differentiation of NSCs into OLs,and different concentrations of PDGF-AA had different effects on differentiation.Conclusion: The combination of bFGF and PDGF-AA can improve the differentiation of NSCs into OLs,with the best concentration being 40 ng/ml PDGF-AA+0.5%FBS. |
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| Keywords: | neural stem cell oligodendrocyte platelet derived growth factor cell differentiation |
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