Humoral mechanisms of regulation of reparative osteogenesis

The effect of the blood serum from animals with active osteogenesis on the biosynthesis of nucleic acids and protein and on mineralization of regenerating bone tissue was studied in experiments in vivo and in vitro. Incorporation of labeled precursors of DNA (³H]thymidine) and protein (¹⁴C]proline) in the recipients was intensified and mineralization of bony callus (incorporation of⁸⁵Sr) was accelerated. Comparison of the order of stimulation of nucleic acid and protein synthesis suggests that the active principle of the serum promotes more rapid cell proliferation in the fracture zone.Laboratory of Pathophysiology and Laboratory of Biochemistry, Research Institute of Experimental and Clinical Orthopedics and Traumatology, Kurgan. (Presented by Academician of the Academy of Medical Sciences of the USSR N. A. Fedorov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 12, pp. 725–727, December, 1977.