雷公藤红素对大鼠血管平滑肌细胞c-myc和血小板源性生长因子的mRNA的影响

目的：观察雷公藤红素对大鼠血管平滑肌细胞（ＶＳＭＣ）中原癌基因（ｃ－ｍｙｃ）和血小板源性生长因子（ＰＤＧＦ）的ｍＲＮＡ表达水平的影响。方法：分别将所培养的第５￣１０代ＶＳＭＣ同步化培养２４ｈ后，加入２０％胎牛血清（ＦＣＳ）和不同剂量的雷公藤红素（０．１、０．２和０．３ｍｇ／Ｌ），共同孵育６ｈ和１２ｈ后，分别提取ＶＳＭＣ胞浆总ＲＮＡ，通过地高辛标记探针，采用斑点杂交方法检测ｃ－ｍｙｃ和ＰＤＧＦ的ｍＲ

Effect of Tripterine on mRNA Expression of c myc and Platelet Derived Growth Factor of Vascular Smooth Muscle Cell in Rats

OBJECTIVE: To observe the effect of Tripterine on mRNA expression of c-myc and platelet derived growth factor (PDGF) in vascular smooth muscle cell (VSMC) of rats. METHODS: 5-10th passage culture of VSMC was used. Tripterine and 20% fetal calf serum were added into the medium of cultured VSMC in concentration of 0.1, 0.2 and 0.3 mg/L after free-serum cultivation for 24 hours. The general RNA was isolated from VSMC at 6 and 12 hours after the drug addition for detection of mRNA expression of c-myc and PDGF respectively by dot blot hybridization. The cDNA probes were labeled by digoxin. RESULTS: Tripterine inhibited the PDGF mRNA expression of VSMC and decreased c-myc mRNA expression in a dose-dependent manner, either vs. control. CONCLUSION: Tripterine could inhibit mRNA expression of c-myc and PDGF in VSMC, therefore, it would inhibit overproliferation of VSMC.