下调Ezrin表达对前列腺癌细胞增殖和运动能力的影响

 目的研究Ezrin蛋白对前列腺癌细胞增殖和运动能力的影响。方法采用前列腺癌PC-3细胞系作为研究对象,针对Ezrin蛋白的编码序列设计小干扰RNA片段,重组为shRNA表达载体转染入细胞,筛选出稳定表达的细胞克隆,RT-PCR和Western blot分别检测Ezrin在基因和蛋白水平表达的变化,用四甲基偶氮唑蓝(MTT)法检测增殖能力,流式细胞术检测细胞周期,Transwell法检测侵袭能力的变化。结果PCR和Western blot显示shRNA对Ezrin在基因和蛋白水平均有显著的下调作用,转染后的前列腺癌细胞生长速度明显减慢,细胞周期显示处于分裂期的细胞比例由(24.58±4.23)%下降到(18.65±2.21)%,处于G1期的细胞数量则由(58.69±3.48)%上升到(66.54±4.13)%。穿过人工基底膜的细胞数量也由(38.6±5.4)减少为(24.5±2.7)(P<0.01)。结论Ezrin对于前列腺癌细胞的增殖和运动能力有重要影响,可能成为肿瘤治疗的重要靶点。

Influence of Down-regulate of Ezrin Expression by RNAi on Proliferation and Invasion of Prostatic Carcinoma

Objective To investigate the effect of Ezrin on the proliferation and invasion of human prostatic carcinoma.Methods Human prostatic carcinoma cell line PC-3 was cultured.A plasmid of a short hairpin RNA targeting Ezrin was constructed,and it was transfected into PC-3 cell line.The expression of Ezrin mRNA and protein were examined by RT-PCR and Western blot method respectively.The proliferation was examined by MTT.Flow cytometry was used to detect the changes of cell cycle.Transwell test was used to detect ...