| 血清HCVRNA荧光定量与丙型肝炎诊断意义 |
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| 引用本文： | 郭振华,景涛,陈青锋,杨彦麟,肖萍.血清HCVRNA荧光定量与丙型肝炎诊断意义[J].中国公共卫生,2005,21(11):1367-1368. |
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| 作者姓名： | 郭振华 景涛 陈青锋 杨彦麟 肖萍 |
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| 作者单位： | 1. 兰州大学第一医院传染病研究所,兰州,730002
2. 兰州大学 |
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| 摘 要： | 目的探讨血清HCVRNA荧光定量在丙型肝炎诊断中的意义。方法采用ELISA法和荧光定量PCR法检测292例慢性丙肝患者抗-HCV和HCVRNA。结果抗-HCV及HCVRNA检出率分别为71.9%(210/292)和51.3%(150/292);抗HCV( )组和抗-HCV(-)组HCVRNA的检出率分别为75.9%(82/108)和10.5%(4/83)。结论抗HCV( )组HCVRNA检出率高于抗-HCV(-)组;血清HCVRNA荧光定量是临床诊断丙型肝炎的直接证据;抗-HCV诊断丙肝感染有漏诊现象。
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| 关 键 词： | 荧光定量 丙型肝炎病毒核糖核酸 丙型肝炎 诊断 |
| 文章编号： | 1001-0580（2005）11-1367-02 |
| 收稿时间： | 2005-02-20 |
| 修稿时间： | 2005年2月20日 |
Significance of fluorescent quantitative serum HCVRNA and diagnoses in hepatitis C |
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| GUO Zhenhua, JING Tao, CHEN Qingfeng.Significance of fluorescent quantitative serum HCVRNA and diagnoses in hepatitis C[J].Chinese Journal of Public Health,2005,21(11):1367-1368. |
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| Authors: | GUO Zhenhua JING Tao CHEN Qingfeng |
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| Institution: | Infection Diseases Institutes, the First Hospital of Lanzhou University, Lanzhou 730000, China |
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| Abstract: | Objective To explore the significance of serum levels of HCVRNA in hepatitis C diagnoses. Methods The anti-HCV was monitored by indirect enzyme-linked immunosorbent assay(ELISA)and serum levels of HCVRNA quantities were tested by fluorescent quantitative PCR(FQ-PCR)in 292 cases of chronic hepatitis C patients. Results The positive rates of anti-HCV and HCVRNA were 71.9%(210/292)and 51.3%(150/292),respectively.The rates of HCVRNA in positive anti-HCV group and negative group were 75.9%(82/108)and 10.5%(4/38),respectively. Conclusion The monitored rate of HCVRNA in positive antiHCV group patients is higher than that of negative group.Serum levels of HCVRNA by FQ-PCR quantities are one of the best direct marks in diagnoses for gepatitis C that could make up for the missed diagnoses with the mark of anti-HCV. |
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| Keywords: | fluorescent quantitative HCV-RNA hepatitis C diagnosis |
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