| Neuropilin-1 在胰腺癌组织及细胞系中的表达 |
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| 引用本文： | 宋海平,王春友,张景辉. Neuropilin-1 在胰腺癌组织及细胞系中的表达[J]. 肿瘤防治研究, 2005, 32(9): 540-550 |
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| 作者姓名： | 宋海平 王春友 张景辉 |
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| 作者单位： | 430022,武汉,华中科技大学协和医院普通外科实验室 |
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| 摘 要： | 目的 了解Neuropilin-1在胰腺癌组织、癌旁组织及胰腺癌细胞系中的表达情况。方法 运用免疫组化法和RT-PCR法分别检测Neuropilin-1及其mRNA在胰腺癌组织、癌旁组织及细胞系中的表达。结果 免疫组化检测发现18例胰腺癌组织标本中Neuropilin-1均呈高表达，而癌旁组织中无一例表达；RT-PCR检测rnRNA的表达结果显示14例胰腺癌组织中有9例表达，癌旁组织中无一例表达，Panc-1、Asps-1、Bxp-3细胞系中均表达。结论 Neuropilin-1表达于大多数胰腺癌组织及细胞系中，而在癌旁组织中不表达，提示Neuropilin-1的表达可能与胰腺癌的发生发展密切相关。
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| 关 键 词： | 胰腺癌 Neuropilin-1 (NRP-1) 血管内皮生长因子(VEGF) |
| 文章编号： | 1000-8578（2005）09-0549-02 |
| 收稿时间： | 2004-09-06 |
| 修稿时间： | 2004-09-062004-12-29 |
Expression of Neuropilin-1 in Human Pancreatic Carcinoma and Cell Lines |
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| SONG Hai-ping,WANG Chun-you,ZHANG Jing-hui. Expression of Neuropilin-1 in Human Pancreatic Carcinoma and Cell Lines[J]. Cancer Research on Prevention and Treatment, 2005, 32(9): 540-550 |
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| Authors: | SONG Hai-ping WANG Chun-you ZHANG Jing-hui |
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| Affiliation: | General Surgery Laboratory of Union hospital of Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Objective To study the expression of Neuropilin-1 in human pancreatic carcinoma specimens and cell lines. Methods We investigated the expression of Neuropilin-1 in human pancreatic carcinoma specimens by immunohistochemical staining and examined the expression of Neuropilin-1 rnRNA in human pancreatic carcinoma specimens and cell lines using RT-PCR. Results Immunohistochemical analysis revealed that NRP-1 was expressed in 14 of 14 human pancreatic adenocarcinoma specimens but was absent in nonmalignant pancreatic tissue. It can be detected the rnRNA of NRP-1 in 9 of 14 human pancreatic carcinoma specimens and Panc-1 ,Aspc-1 ,Bxpc-3 cell lines while can not be detected in nonmalignant pancreatic specimens. Conclusion Neuropilin-1 was expressed in most of human pancreatic carcinoma specimens and cell lines but not expressed in nonmalignant pancreatic tissue. It suggested the the expression of Neuropilin- 1 may be relevant to the risk and development of pancreatic carcinoma. |
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| Keywords: | Neuropilin-1(NRP-1) |
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