武汉地区汉人2型糖尿病合并肥胖及非肥胖患者Lepr基因变异的对比性分析

目的研究瘦素受体基因GIN223Arg与2型糖尿病合并肥胖的关系。方法运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定无亲缘关系且具有完整资料的武汉地区汉人351例(其中2型糖尿病合并肥胖组131例、2型糖尿病非肥胖组148例、正常对照组72例)。结果①2型糖尿病合并肥胖组基因型AA、AG、GG(分别为0.398、0.480和0.122)与2型糖尿病非肥胖组的(0.196、0.223和0.581)和正常对照组分别为(0.208、0.264和0.528)比较有显著性差异(P<0.01)。②2型糖尿病合并肥胖组等位基因分布频率A、G分别为0.642及0.358,与非肥胖组及正常对照组比较有统计学意义(P<0.01)。③应用多重回归分析(NominalRegression)2型糖尿病与临床各变量的关系发现,2型糖尿病GIN223Arg变异(由A突变G)与BMI、收缩压、舒张压(分别为P=0.001,P=0.032,P=0.037)相关。结论瘦素受体基因GIN223Arg变异可能与2型糖尿病合并肥胖和高血压相关。

Comparison of Lepr Gene Variant between Patients with Obesity and ones without Obesity in the Hans with Type 2 Diabetes Mellitus in Wuhan District

Objective To investigate the relationship between Gln 223 Arg variant ,a leptin receptor gene and obesity accompanied with type 2 diabetes mellitus. Methods The genotypes of Gln223Arg variant were determined by PCR-RFLP in 351 unrelated subjects of Wuhan "Han" population who included 72 subjects with normal glucose tolerance as control, 131 with obesity in type 2 diabetic patients and148 without obesity in type 2 diabetic patients). The clinical data were also analyzed. Results In patients with obesity in type 2 diabetic patients, the genotype frequency was AA, AG and GG(0.398,0480 and 0.122 respectively), while in patients without obesity in type 2 diabetic patients, the genotype frequency was AA, AG and GG(0.196 , 0.223 and 0.581 respectively), and in control it was AG, GG and AA(0.208 .0.264and 0.528 respectively). There were significant differences in the genotype frequency of Gln223 Arg variant in leptin receptor gene among the three groups (P<0.001). In the obesity in type 2 diabetic patients "A" and "G" allele were 0. 642 and 0.358 respectively , which were significantly different from those without obesity in type 2 diabetic patients and control subjects (P<0.01,P<0.001). Logistic regression analysis showed that this gene variant was an independent risk factor of type 2 diabetic patients accompanied with obesity. Conclusion Gln 223 Arg variant in leptin receptor gene is associated with obesity and hypertension in type 2 diabetic patients