芝麻素对肾性高血压伴高血脂大鼠肾脏的保护作用研究

目的： 观察芝麻素对肾性高血压伴高血脂大鼠肾脏的保护作用。方法：制备两肾一夹型肾性高血压伴高血脂大鼠模型(RHHR)，灌胃给予不同剂量的芝麻素7周后，测定各组血清肌酐（Scr）、血尿素氮（BUN）、24 h 尿蛋白含量（UPE）和肾组织匀浆中抗氧化酶的活性，包括总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、总NOS（TNOS）、诱导型NOS（iNOS）、结构型NOS（cNOS）和抑制羟自由基能力及肾组织匀浆中丙二醛（MDA）、过氧化氢（H2O2）及一氧化氮（NO）含量。结果：高、中（100 mg/kg、33 mg/kg）剂量组芝麻素可抑制RHHR大鼠血清Scr、BUN和尿UPE水平升高（P<0.01）。与模型组比较可提高肾组织匀浆NO、cNOS、SOD、T-AOC和抑制羟自由基能力（P<0.01或P<0.05），降低MDA、iNOS 、H2O2（P<0.01或P<0.05）含量。结论：芝麻素对肾性高血压伴高血脂大鼠肾脏具有保护作用，其机制与抗氧化应激、清除自由基和抑制iNOS活性等有关。

Renoprotective effects of sesamin on renal hypertensive and hyperlipidemic rats

AIM: To investigate the effects of sesamin on progression of renal injury in renal hypertensive and hyperlipidemic rats (RHHR). METHODS: RHHR was induced by 2K1C and high lipid baitvessel. After 7 weeks of intragastric administration with sesamin, the contents of serum creatinine (Scr), blood urea nitrogen (BUN), 24 h urinary protein excretion (UPE) were measured. In addition, the activity of total antioxidative capacity (T-AOC), superoxide dismutase (SOD), the concentrations of malondialdehyde (MDA), hydrogen peroxide (H2O2), and the nitric oxide synthase (NOS) and nitric oxide (NO) levels in renal homogenate were measured. RESULTS: Compared with the model group, seasamin (in 100 mg·kg-1 and 33 mg·kg-1 groups) evidently decreased the contents of Scr, BUN, UP and the concentration of MDA, iNOS, H2O2 in renal tissure. It also improved the levels of NO, cNOS and activity of SOD, T-AOC in renal tissure. CONCLUSION: Sesamin ameliorates hypertensive and hyperlipidemic-induced renal injury, probably by enhancing antioxidative activity, scavenging hydroxyl radical and restraining iNOS level.