| hBMP2转基因载体的构建及其在兔骨髓基质细胞中的表达 |
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| 引用本文： | 陈峻江,刘丹平,崔大祥,王伟,张正,郭韬,张男. hBMP2转基因载体的构建及其在兔骨髓基质细胞中的表达[J]. 实验动物与比较医学, 2006, 26(1): 44-46 |
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| 作者姓名： | 陈峻江 刘丹平 崔大祥 王伟 张正 郭韬 张男 |
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| 作者单位： | 中国医学科学院,中国协和医科大学医学生物学研究所,昆明,650118 |
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| 摘 要： | 腹泻是离乳婴猴常见病,死亡率极高的病。作者多年的统计结果表明,死亡婴猴中58．3％死于腹泻。过去腹泻早期也使用过一些药物进行治疗, 但效果不太理想。最近,作者用胃肠粘膜保护剂联合思密达,口服补液盐辅助治疗婴猴腹泻,取得良好效果。 1 材料与方法 1．1 动物的选择选取离乳后3-60 d(4月龄离乳)的腹泻婴猴作试验观察,发病时间不超过24 h。全部婴猴半屏
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| 关 键 词： | 口服补液盐 联合治疗 腹泻 疗效观察 密达 猴 胃肠粘膜 辅助治疗 常见病 死亡率 |
| 文章编号： | 1004-8448(2006)01-0044-01 |
| 收稿时间： | 2005-09-12 |
| 修稿时间： | 2005-09-12 |
Construction of pcDNA3-hBMP2 Vector and Its Expression in Rabbit Bone Marrow Stromal Cells |
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| CHEN Jun-jiang,LIU Dan-ping,CUI Da-xiang,WANG Wei,ZHANG Zheng,GUO Tao,ZHANG Nan. Construction of pcDNA3-hBMP2 Vector and Its Expression in Rabbit Bone Marrow Stromal Cells[J]. Laboratory Animal and Comparative Medicine, 2006, 26(1): 44-46 |
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| Authors: | CHEN Jun-jiang LIU Dan-ping CUI Da-xiang WANG Wei ZHANG Zheng GUO Tao ZHANG Nan |
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| Abstract: | Objective To construct human bone morphogenetic protein-2(hBMP-2 ) expressing eukaryotic vector(pcDNA3-hBMP2) and examine its expression in rabbit bone marrow stromal cells(MSCs).Methods pcDNA3-hBMP2 vector was constructed using gene clone and recombination technique.After being confirmed by enzyme cut,pcDNA3-hBMP2 was transfected into cultured rabbit MSCs in vitro with the help of liposome reagent FuGENE6.The expression of hBMP-2 was determined by in situ hybridization and immunohistochemical analysis.Results The pcDNA3-hBMP2 vector was constructed successfully.The rabbit MSCs transfected by pcDNA3-hBMP2 showed transient and stable expression of BMP2.Conclusion pcDNA3-hBMP2 can express hBMP2 in bone marrow stromal cells. |
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| Keywords: | hBMP2 |
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