miR-144和miR-502—3p在原发性肝细胞癌术后复发患者中的表达及临床意义

目的探讨miR-144和miR-502-3p在原发性肝细胞癌术后复发患者中的表达及临床意义。方法从我院肝癌数据标本库中挑选82例原发性肝癌深低温冻存组织标本。其中50例早期复发组（术后1年内肝内出现复发病灶）；32例非早期复发组（术后超过2年未出现肝内复发病灶）。运用实时荧光定量PCR验证miR-144、miR-502—3p在两组肝癌组织中的表达水平。结果相对于非早期复发组，miR-144在肝癌早期复发组中表达上调（43．893±107．890）VS（6．321±6．845），P=0．018]；其在肝癌组织中的表达仅与肝癌术后早期复发有关（P〈0．05）。而miR-502—3p在肝癌早期复发组中表达下调（6．702±9．775）VS（26．467±39．613），P=0．009]；其在肝癌组织中的表达与肿瘤直径、肝癌术后早期复发、脉管癌栓、肝硬化、Edmonson病理分级有关（P〈0．05）。结论miR-144、miR-502-3p与肝癌的早期复发转移密切相关，并且可能是肝癌早期复发的分子标记物以及未来肝癌靶向治疗的有效靶点。

Expression and clinical significance of miR-144 and miR-502-3p with early recurrence after radical surgery in primary hepatocellular carcinoma

Objective To investigate the expression and clinical significance of miR-144 and miR-502-3p with early recurrence after radical surgery in primary hepatocellular carcinoma. Methods 82 HCC cryopreservation tissue samples from the liver carcinoma specimens data bank in our hospital obtained after radical surgery were divided into 2 groups： early recurrence group （50 eases） and non-early recurrence group （32 cases）. A quantitative real-time polymerase chain reaction （qRT-PCR） was performed to detect the expression of miR-144 and mir-502-3p. Results Compared with the non-early recurrence group, the expression of miR-144 was up-regulated in early recurrence group （43.893±107.890） vs （6.321±6.845）,P=0.O18]. The expression of miR-144 was only correlated with early recurrence after radical surgery （P〈0.05）, while the expression of miR-502-3p was down-regulated but without statistical significance （6.702±9.775） vs （26.467±39.613）,P=0.009]. The expression of miR-502-3p was correlated to tumor diameter, reeurrence in HCC, vascular invasion, cirrhosis of liver, and Edmonson pathologieal grading（P〈0.05）. Conclusions mir-144 and mir-502- 3p are closely related to early liver cancer recurrence and metastasis, mir-144 and mir-502-3p may be a molecular marker of HCC early recurrence, as well as a target for future targeted therapy of liver cancer.