吲哚-3-甲醇对人肝癌细胞株SMMC-7721增殖和凋亡的影响

目的探讨吲哚-3-甲醇（13C）对人肝癌细胞株SMMC．7721的增殖和凋亡的影响。方法不同浓度的13C（100、150、200、250、300、350Ixmol／L）处理细胞48h后，显微镜下观察细胞生长状况；采用WST-1法检测细胞增殖情况，Hoehest33258染色、TUNEL染色检测细胞凋亡情况，Westernblot法检测凋亡相关蛋白。结果13C能够抑制SMMC．7721细胞增殖，诱导细胞凋亡，处理浓度在250μmol／L以下时，细胞生长变慢；处理浓度达到300μmol／L时，大量细胞凋亡。13C浓度高于200μmol／L时CytC、Cleavedcaspase．9和Cleavedcaspase．3蛋白表达明显增加，且随着13C浓度的增加三种蛋白的表达也明显增加。结论13C在体外实验条件下可抑制人肝癌细胞株SMMC．7721增殖并诱导其发生凋亡。

Effects of indole-3-carbinol on the proliferation and apoptosis of human liver cancer cell line SMMC-7721

Objective To investigate the effects of indole-3-carbinol on the proliferation and apoptosis of human liver cancer cell line SMMC-7721. Methods The growth status of SMMC-7721 was observed under Microscope. The inhibition of cell proliferation was assayed by WST-1 agent, and the apoptosis was shown with Hochest33258 and TUNEL staining. The apoptosis related protein were detected by Western blot. Results I3C could inhibit the proliferation of SMMC-7721 and induced the apoptosis in a dose-dependent manner. The cell proliferation was slow at doses below 250 μmol/L of I3C. And a large proportion of apoptotic cells were observed at doses above 300 μmol/L of I3C. Furthermore , the expressionsof Cyt C, Cleaved caspase-9 and Cleaved caspase-3 increased significantly when I3C at concentrations higher than 200μ mol/L.With the increase of I3C concentration, three kinds of protein expression also increased significantly. Conclusion I3C could significantly inhibit the human liver cancer cell proliferation and induce apoptosis in a dose dependent manner.