电针猫“足三里”穴抑制胃扩张所致的一过性下食管括约肌松弛

目的:探讨电针"足三里"穴以及"足三里"联合"内关"穴对一过性下食管括约肌松弛(TLESR)的影响及其机制。方法:健康成年猫随机分为8组:足三里组、内关组、足三里联合内关组(简称联合组)、非经非穴组、单纯胃扩张组(模型组)、生理盐水组、L-精氨酸组、他克林组,每组5只。通过逐次向胃内注气的方法建立胃扩张诱发TLESR的动物模型。各治疗组在胃扩张的同时给予电针刺激,持续60min。用低顺应性毛细管灌注测压系统记录、分析TLESR频率、共腔发生率和下食管括约肌压力(LESP)。结果:与模型组相比,足三里组、内关组和联合组均可以显著降低TLESR的频率及其合并共腔的比率(P<0.05);与电针前相比,电针"足三里"穴可以显著增加LESP(P<0.05),其它组与电针前相比差异无统计学意义(P>0.05);他克林可以逆转电针"足三里"穴对TLESR的抑制,而L-精氨酸则不能逆转。结论:电针"足三里"能够减少胃扩张引起的TLESR频率的增多和共腔的发生,此作用的发挥可能与胆碱通路有关。

Electroacupuncture at "Zusanli" (ST 36) Can Inhibit Frequencies of Transient Lower Esophageal Sphincter Relaxation Induced by Gastric Distention in Cats

Objective To observe the influence of electroacupuncture(EA) of "Zusanli"(ST 36) and ST 36 + "Neiguan"(PC 6) on gastric distention induced transient lower esophageal sphincter relaxation(TLESR) in cats.Methods Forty cats were randomly divided into model(gastric distension) group,EA-ST 36 group,EA-PC 6 group,EA-ST 36+PC 6 group,EA-non-acupoint group,EA-ST 36+saline(2mL i.v.) group,EA-ST 36+L-arginine(precusor of nitric oxide,200mg/kg i.v.) group,and EA-ST 36+Tacrine(cholinesterase inhibitor,5.6mg/kg i.p.) group,with 5 cats being in each group.TLESR model was established by extending the stomach with an air balloon.EA(2Hz/100Hz,3-4mA) was applied to bilateral ST 36,PC 6 and ST 36+PC 6 and non-acupoint(exterior-superior site of the hip) while gastric distension.L-Arginine was given 10min before EA,and Tacrine was given 30 min before EA.The frequency of TLESR and lower esophageal sphincter pressure(LESP) were recorded by using a perfused sleeve assembly.Results Compared with the model group,the frequency of TLESR in 60min as well as the percentage of common cavity during TLESR were significantly decreased in the EA-ST 36,EA-PC 6,EA-ST 36+PC 6,EA-ST 36+saline groups(P<0.05).Compared with the EA-ST 36+saline group,the frequency of TLESR in 60 min was up-regulated significantly in the EA-ST 36+Tacrine group(P<0.01) rather than in the EA-ST 36+L-Arginine group,suggesting a reduction of the efficacy of EA-ST 36 by cholinergic suppression(not by nitric oxide).No significant differences were found among the EA-ST 36,EA-PC 6 and EA-ST 36+PC 6 groups in the frequency of TLESR and the percentage of common cavity(P>0.05).In comparison with the baseline level,LESP was increased significantly only in the EA-ST 36 group(P<0.05).Conclusion EA at ST 36 can reduce both the frequency of TLESR and the percentage of common cavity during TLESR in gastric distension cats,which may be mediated by cholinergic pathway.