肺炎衣原体包涵体膜蛋白Cpn0147抗原性分析

目的:在大肠杆菌中表达纯化融合蛋白GST-Cpn0147,研究其免疫原性。方法:将Cpn0147基因克隆到原核表达载体PGEX-6P2,转化入大肠杆菌XL-Blue,经IPTG诱导表达并纯化;以纯化的融合蛋白免疫BALB/c小鼠,制备免疫血清,ELISA法证实其免疫原性并检测免疫血清的效价,Western-blot检测免疫反应性。结果:在大肠杆菌中表达了GST-Cpn0147融合蛋白,以该蛋白免疫小鼠获得了免疫血清。结论:获得了GST-Cpn0147融合蛋白,并证实其具有免疫原性和免疫反应性。

Antigenicity Analysis of Inclusion Body Membrane Protein Cpn0147 of Chlamydia Pneumoniae

Objective To express and purify recombinant protein GST-Cpn0147 and to explore its antigenicity.Methods The gene Cpn0147 was cloned into prokaryotic expression vector PGEX-6P2.PGEX-6P2-Cpn0147 was introduced into E.coil XL-Blue and fusion protein GST-Cpn0147 was expressed and purified.Then the purified fusion protein was used to vaccinate Balb/c mice,protein Cpn0147 immunogenicity was detected by ELISA and its immunity responsibility was detected by Western-blot.Results The fusion protein GST-Cpn0147 was successfully expressed in E.coil,serum antibody was obtained from vaccinated mice.Conclusion The fusion protein GST-Cpn0147 was obtained and its immunogenicity and immunoreactivity were proved.