单胚胎玻璃化冷冻对小鼠胚胎体外发育的影响

目的:比较单胚胎玻璃化冷冻与慢速冷冻对小鼠分裂期胚胎损伤和体外发育的影响,探讨使用玻璃化冷冻保存大量胚胎的可行性。方法:选择8-细胞胚胎随机进行改良的玻璃化和慢速冷冻,复苏后培养至囊胚孵出,计数胚胎复苏率、卵裂球死亡率、囊胚形成率、细胞团数、孵出率,并进行组间比较分析。结果:玻璃化冷冻组复苏率、卵裂球死亡率、囊胚形成率、孵出率、细胞团数分别为100%、12.7%、75.7%、66.4%、112,慢速冷冻组分别为87.1%、31.0%、35.5%、58.7%、78,除囊胚孵出率其余各指标两组间差异均具有统计学意义。结论:单胚胎玻璃化法冻存小鼠分裂期胚胎复苏后细胞成活率高,结构完整,更好的保持了胚胎的发育潜能。

Effects of Single-embryo Vitrification on the Cleavage Stage Embryos Developmental Ability in vitro

Objective To compare the effect between single-embryo vitrification and slow-freezing on development of mice cleavage stage embryos in vitro,and to explore whether that vitrification is feasible to freeze preserve a great quantity of embryos.Methods Mice 8-cell embryos were cryopreserved by vitrification or slow-freezing randomly and cultured until the blastocyst hatched out after thawed.The embryo recovery rate,blastomere mortality,blastocyst formation rate,the number of cell clusters,hatched rate were counted and statistically analyzed.Results In the vitrification group,the recovery rate,blastomere mortality,blastocyst formation rate,hatched rate,the number of cell clusters were 100%,12.7%,75.7%,66.4%,112,respectively.While they were 87.1%,31.0%,35.5%,58.7%,78 in slow freezing group,respectively.All these indexes were significantly difference between two groups excluding the hatched rate.Conclusion The survival rate of mice cleavage stage embryos cryopreserved by single-embryo vitrification were high,the structure integrality and developmental abillity of embryos could be better maintained by this method.