不同部位耳针对大鼠癫痫发作的抑制效应

目的:观察不同部位耳针对戊四氮致癫痫大鼠发作的抑制效应。方法:SD大鼠随机分为模型组、耳尖组、耳外缘组、耳垂组、耳甲艇组、耳甲腔组,每组各10只。将直径为50μm的金属微电极移植到大鼠躯体感觉皮层,待大鼠清醒并恢复后进行实验。腹腔注射戊四氮(60mg/kg)造成大鼠癫痫模型。除模型组外,其余各组分别给予外耳不同部位电针刺激30min。观察第1次癫痫发作潜伏期;癫痫发作行为学评分根据Racine分级标准进行;癫痫发作根据皮层体感区场电位的幅度(超过基础值的5倍)确定,记录60s内癫痫发作总的持续时间。结果:与模型组比较,除耳尖组外其它各耳针治疗组大鼠癫痫发作潜伏期延长(均P<0.01);与其它治疗组相比,耳甲艇组和耳甲腔组大鼠癫痫发作潜伏期延长(均P<0.01)。与模型组比较,各耳针治疗组大鼠癫痫发作行为学评分和癫痫发作时间均减少(均P<0.01);与其它治疗组相比,耳甲艇组和耳甲腔组大鼠癫痫发作行为学评分和癫痫发作时间均减少(均P<0.01)。耳甲艇组和耳甲腔组大鼠第1次癫痫发作潜伏期、行为学评分和癫痫发作时间的差异无统计学意义(均P>0.05)。结论:耳针能抑制大鼠癫痫发作,电针耳甲(包括耳甲腔和耳甲艇)抑制癫痫发作的效应较电针外耳其它部位显著,这可能与刺激了迷走神经耳支有关。

Effect of Electroacupuncture of Different Regions of the Auricle on Epileptic Seizures in Epilepsy Rats

Objective To observe the anti-seizure effect of electroacupuncture(EA) at differentsites regions of the auricle in awake rats.Methods Sixty male SD rats were randomly divided into model,ear-apex,ear-outer-margin,earlobe,cymba-concha,cavitas-conchae groups(n=10 in each group).A metal microelectrode(diameter: 50μm) was implanted into the somatosensary cortex for recording the field potentials(FPs).Epileptic seizure model was established by intraperitoneal injection of pentylenetetrazol(PTZ,60mg/kg).EA(20Hz,1mA) was applied to ear-apex,ear-outer-margin,earlobe,cymaba-concha and cavitas-conchae for 30min,respectively.The epileptic behavior changes(scores) were determined in the light of Racine's method,and the epileptic seizure was assessed according to the epileptic FPs(5-folds above the basic amplitude) of the cerebral cortex.Results In comparison with the model group,the latency of the first seizure of epilepsy in the ear-outer-margin,earlobe,cymba-concha,cavitas-conchae groups were increased significantly(P<0.01),while the scores of epileptic behavior and the duration of seizure shown by FPs decreased considerably in the ear-apex,ear-outer-margin,earlobe,cymba-concha,cavitas-conchae groups(P<0.01).Comparison among the five EA treatment groups showed that the effects of both cymba-concha and cavitas-conchae groups were significantly superior to those of ear-apex,earlobe and ear-outer-margin groups in increasing the latency of the first seizure of epilepsy,and reducing the scores of the epileptic behavior and seizure duration(P<0.01).No significant differences were found between the cymba-concha and cavitas-conchae groups in the above-mentioned 3 indexes(P>0.05).Conclusion EA of cymba-concha and cavitas-conchae has a good effect in suppressing epileptic seizures,which may be mediated via the auricular branch of the vagus nerve.