肝癥口服液对大鼠实验性肝癌的预防作用及对肝组织细胞黏附分子-1表达的影响

目的探讨肝癥口服液对二乙基亚硝胺诱发大鼠肝癌的预防作用及对肝组织细胞黏附分子-1(ICAM-1)表达的影响。方法将75只大鼠随机分为4组,正常对照组15只,模型组、中药大剂量预防组、中药小剂量预防组各20只。除正常对照组外,给予大鼠二乙基亚硝胺(DEN)35mg/kg,灌胃,2次/周,共16周,预防组同时给予药物预防,16周后所有实验组停用DEN,各药物预防组继续用药至20周末实验结束,于第20周末处死大鼠,分别进行:①肝组织切片常规HE染色,观察形态变化;②免疫组化方法观察ICAM-1在肝组织中的表达。结果模型组、肝癥口服液大剂量预防组、小剂量预防组大鼠肝癌发生率分别为88.8%、23.08%、30.00%,预防组肝癌发生率显著低于模型组(P<0.05,P<0.01),模型组ICAM-1免疫组化染色的灰度值显著低于各药物预防组(P<0.05,P<0.01)。结论①肝癥口服液能有效地预防大鼠实验性肝癌的发生。②肝癥口服液在大鼠癌变形成过程中下调肝组织中ICAM-1的表达。

The effect of Ganzheng solution on the ICAM- 1 expression in liver tissues of rat in the diethylnitrosamine induced hepatocarcinogenesis

Objective To investigate the effect of Ganzheng peroral solution on the ICAM-1 expression in liver tissues of rat in the diethylnitrosamine induced hepatocarcinogenesis. Methods 75 male rats were randomly divided into four groups,i.e.,normal control group, model group, large dose Ganzheng solution group, small dose Ganzheng solution group , except for normal control, rats of three groups were fed with DEN water solution 35 mg/kg twice a week for 16 weeks, prophylactic drugs were used similarly. DEN was stopped to use in all groups after 16 weeks, all rats were sacrificed at 20 th experimental weeks. The histological and ultrastructural changes in liver tissues were observed by Hematoxylin-eosin (HE) staining; ICAM-1 expression in liver tissues were also used to compare the effect of the drugs mentioned above. Results 1. By the end of the 20th experimental week, the incidence of HCC was 88.8%, 23.08%, 30% in model group, large dose Ganzheng solution group and small dose Ganzheng solution group respectively. The tumor incidence of model group was significantly higher than that of other groups (P<0.05 or P<0.01). 2. Image analysis indicated that the Grey values of model group obviously decreased much more and significantly lower than those of prophylactic groups (P<0.05 or P<0.01). Conclusions 1.The results indicated that Ganzheng solution exhibited repressive effect on the genesis of HCC in rat. 2. Gan-Zhen solution can down-regulate the expressions of ICAM-1 in liver tissues during diethylnitrosamine induced hepatocarcinigenesis.