反相高效液相色谱法测定无花果中游离氨基酸的含量

目的　测定无花果中游离氨基酸的含量。方法　用OPA与 2 -ME作衍生剂 ,在梯度洗脱、荧光检测下 (λex =335nm ,λem =4 30nm) ,采用反相高效液相色谱法测定 15种游离氨基酸的含量。结果　无花果果实中含有多种人体必须氨基酸 ,其含量占总游离氨基酸的4 0 .2 0 %。其中 ,丙氨酸、丝氨酸、天门冬氨酸和赖氨酸含量较高。结论　无花果果实是富含氨基酸的物质 ,具有较高的食用价值和保健作用

Determination of free amino acid contents in Ficus carica by RP-HPLC

Objective To determine the contents of free amino acids in fresh Ficus carica fruit.Methods Concentrations of 15 kinds of amino acids in Ficus carica fruit were determined by reversed-phase high performance liquid chromatography(RP-HPLC),with OPA and 2-mercapyo ethanol as precolumn derivative reagents.Gradient elution and fluorescence detector was used(λex=335nm,λem=430nm).Results Fifteen kinds of amino acids had been found, and there were many kinds of essential amino acids needed by human body in Ficus carica fruit.The contents of essential amino acids in the total free amino acids were 45.06% and concentrations of Ala, Ser, Lys and Asp were slightly higher.Conclusion Amino acids are rich in Ficus carica fruit which is valuable as special diet.