白藜芦醇对TRAIL诱导人肝癌细胞SMMC-7721凋亡的影响研究

目的研究肿瘤坏死因子相关凋亡诱导配体（TRAIL）诱导人肝癌细胞株SMMC-7721凋亡作用及白藜芦醇对TRAIL的增敏效果。方法培养SMMC-7721细胞，同步化后，分成对照组、TRAIL干预组、Res＋TRAIL干预组，作用24h后，收集细胞，用流式细胞仪进行凋亡分析。结果对照组SMMC-7721细胞的凋亡率为3．02％±0．17％，TRAIL干预组凋亡率为8．55％±1．46％，Res＋TRAIl。干预组中25μmol·l-1、50μmol·l-1、100μmol·l-1。凋亡率分别为8．61％±1．41％、20．72％±1．33％、27．19％±1．43％。TRAlL干预组、Res＋TRAIL干预组与对照组相比，差异均有显著性（P〈0．01）。Res＋TRAIL干预组（5μmol·l-1、100μmol·l-1白藜芦醇）与TRAIL干预组相比，差异均有显著性（P〈0．01）；而Res＋TRAIL干预组（25μmol·l-1白藜芦醇）与TRAIL干预组比较差别无显著性（P〉0．05）。结论本研究表明’FRAIL对SMMC-7721细胞有凋亡诱导作用；白藜芦醇对TRAIL诱导人肝癌细胞SMMC-7721凋亡有致敏作用，且呈剂量依赖关系。

The study of the effects of resveratrol on TRAIL-induced apoptosis in human hepatoma cell line SMMC-7721

Objective The aim of our study was to observe the effect of tumor necrosis factor-related apoptosis-inducing ligand （TRAIL） on inducing apoptosis of human hepatoma SMMC-7721 cells,and res- veratrol （Res） executing the role in the process. Methods SMMC-7721 cells were cultured in vitro. After being synchronized, the cells were divided into three gropus： control group,TRAIL intervention group, Res and TRAIl, intervention group. The flow cytometry （ FCM ） was given to detect the effects of Res after the treatment with TRAIL/ Res and TRAIL for 24 hours. Results The apoptotie rate of SMMC-7721 cells of control group was 3.02% ±0. 17%. The apoptotie rate was 8.55% ± 1.46% , with 100ng·ml -1 TRAIL. The apoptotic rates were 28. 61% ± 1.41% ,20. 72%± 1.33% ,27.19% ± 1.43% ,respectively, with the supplement of 25μmol·l-1 or 50μmol·l-1 or 100μmol·l-1Res. The apoptotic index of SMMC-7721 cells in TRAIl, intervention group was increased compared with control group （P 〈0. 01 ）. Among res and TRAIL intervention group, the apoptotic index in 50μmol·l-1 and 100μmol·l-1 Res intervention group was increased compared with TRAIl, intervention group （P 〈 0. 01 ）. But there no difference between 25μmol·l-1 Res inter- vention group and TRAIL intervention group （ P 〉 0. 05 ）. Conclusions TRAIL can induce apoptosis of SMMC-7721 cells in vitro. Res can enhance TRAIL-induced apoptosis of SMMC-7721 cells in dose-depend- ent manner.