黄芩和黄芩苷对幽门螺杆菌的体外抗菌活性研究

目的：比较黄芩乙醇提取物与黄芩苷对幽门螺杆菌（H.pylori）的体外抗菌活性。方法：使用热回流提取法从黄芩粗粉中制备黄芩乙醇提取物,并从黄芩粗粉中提取精制黄芩苷。经高效液相色谱（HPLC）鉴定精制黄芩苷的纯度。通过体外药敏试验判断黄芩乙醇提取物和黄芩苷对H.pylori的抗菌活性。结果：从100 g黄芩粗粉中提取精制得黄芩苷5.01 g,经HPLC测定精制黄芩苷得纯度为96.8%。通过液体倍比稀释药敏试验测定黄芩乙醇提取物和黄芩苷对H.pylori的抑菌效果。黄芩苷对10株H.pylori的MIC50为1.04 mg/ml,MIC90为1.30 mg/ml;黄芩乙醇提取物对10株H.pylori的MIC50为2.60 mg/ml,MIC90为3.26 mg/ml。结论：黄芩乙醇提取物与黄芩苷均有较好的体外抗H.pylori活性,黄芩苷对H.pylori的抑菌效果优于黄芩乙醇提取物。

Study of Scutellaria baicalensis and Baicalin against Antimicrobial Susceptibility of Helicobacter pylori Strains in vitro

OBJECTIVE: To compare the antimicrobial activities of Scutellaria baicalensis and Baicalin against Helicobacter pylori (H. pylori) in vitro. METHODS: The crude alcohol extraction of Scutellaria baicalensis was obtained by successive extractions with ethanol. Baicalin was extracted by using organic extraction methods and the purity was analyzed by high-performance liquid chromatography (HPLC). The crude alcohol extraction of Scutellaria baicalensis and Baicalin were used in broth dilution assays to test for antibacterial properties. RESULTS: The content of Baicalin was 5.01 g from 100 g Scutellaria baicalensis. The purity of Baicalin was 96.8%. The crude alcohol extraction of Scutellaria baicalensis and Baicalin were tested for their ability to inhibit H. pylori in vitro by using broth dilution assays. The MIC50 and MIC90 of Baicalin against ten strains of H. pylori were 1.04 and 1.30 mg/ml respectively. The MIC50 and MIC90 of the crude product against ten strains of H. pylori were 2.60 and 3.26 mg/ml respectively. CONCLUSION: The Baicalin and Scutellaria baicalensis are bactericidal against H. pylori. The antimicrobial activity of Baicalin is greater than that of Scutellaria baicalensis.