藤梨根提取物通过调控miR-192-5p/ARPP19轴影响结直肠癌细胞的增殖和凋亡

背景 藤梨根提取物(rattan root extract,RRE)可抑制胃癌、肺癌等肿瘤细胞增殖,具有一定抗肿瘤作用,但其是否影响结直肠癌细胞的恶性表型还未知.miR-192-5p在结直肠癌组织中表达降低,且其低表达与肿瘤大小等临床病理特征相关,可作为结直肠癌诊治的潜在生物学标志物.StarBase生物信息学软件预测...

Radix Actinidiae extract affects proliferation and apoptosis of colorectal cancer cells by regulating miR-192-5p/ARPP19

BACKGROUND Radix Actinidiae extract(RAE)can inhibit the proliferation of gastric cancer,lung cancer,and other tumor cells,and has appreciated anti-tumor effects,but it is unknown whether it affects the malignant phenotype of colorectal cancer cells.The expression of miR-192-5p is reduced in colorectal cancer tissues,and its low expression is related to clinicopathological characteristics such as tumor size.Thus,it can be used as a potential biomarker for the diagnosis and treatment of colorectal cancer.StarBase bioinformatics software predicts that cAMP-regulated phosphoprotein 19(ARPP19)may be a target gene of miR-192-5p.This study hypothesized that RAE can affect the proliferation and apoptosis of colorectal cancer cells by regulating the miR-192-5p/ARPP19 axis.AIM To investigate the effects and mechanism of RAE on proliferation and apoptosis of colorectal cancer SW480 cells.METHODS After SW480 cells were treated with RAE,cell proliferation was detected by MTT assay,apoptosis was detected by flow cytometry,and the protein expression levels of Cyclin D1,cleaved Caspase-3,and ARPP19 were detected by Western blot.The expression of miR-192-5p and ARPP19 mRNA in cells was detected by RTqPCR.miR-192-5p mimic or ARPP19 small interfering RNA was transfected into SW480 cells,and then the above methods were used to observe the effects of overexpressing miR-192-5p or inhibiting ARPP19 on cell proliferation,apoptosis,and the protein expression of CyclinD1 and cleaved Caspase-3 in SW480 cells.The regulatory relationship between miR-192-5p and ARPP19 was verified by the dual luciferase reporter gene assay.RESULTS RAE decreased the survival rate of SW480 cells and the expression of ARPP19 mRNA and protein(P<0.05),but increased the apoptosis rate of SW480 cells and the expression of cleaved Caspase-3 protein and miR-192-5p(P<0.05).Over-expressing miR-192-5p or inhibiting ARPP19 expression could decrease the survival rate of SW480 cells and the expression of CyclinD1 protein(P<0.05),but increase the apoptosis rate of SW480 cells and the expression of cleaved Caspase-3 protein(P<0.05).miR-192-5p negatively regulated the expression of ARPP19 in SW480 cells.Inhibiting miR-192-5p reversed the effects of RAE on proliferation,apoptosis,and the protein expression of CyclinD1 and cleaved Caspase-3 in SW480 cells.Inhibiting ARPP19 reversed the effects of inhibiting miR-192-5p on proliferation,apoptosis,and the protein expression of CyclinD1 and cleaved Caspase-3 of SW480 cells treated with RAE.CONCLUSION RAE inhibits the proliferation of colorectal cancer SW480 cells and promotes apoptosis,and its mechanism of action is related to the regulation of the miR-192-5p/ARPP19 axis.