抗α1-肾上腺素能受体抗体对培养大鼠主动脉平滑肌细胞增殖的影响

目的：观察抗α1-肾上腺素能受体自身抗体对培养平滑肌细胞增殖的影响，并探讨其可能机制。 方法： 用酶消化法培养大鼠平滑肌细胞及用亲和层析的方法纯化抗α1-肾上腺素能受体抗体，用BrdU掺入法检测平滑肌细胞的增殖率，用RT-PCR及Western blotting法检测c-jun、c-fos的表达。 结果： 抗α1-肾上腺素能受体抗体可以显著增加VSMC的增殖，并随时间的延长及抗体滴度的增加而增强，并可增加c-jun mRNA和蛋白的表达，其作用与NE相似，均显著高于正常对照IgG，并均可被α1-肾上腺素能受体阻滞剂prazosin阻断；而该抗体对c-fos的表达无显著增加。 结论： 抗α1-肾上腺素能受体抗体可以显著增加培养大鼠平滑肌细胞的增殖，并可增加c-jun的表达，在高血压血管重塑中可能发挥一定作用。

Effects of the antibodies against α1 - adrenergic receptor on the proliferation of vascular smooth muscle cells in rats

AIM: The autoantibodies against α1-adrenergic receptor that was found in patients with malignant hypertension, primary hypertension and refractory hypertension has the agonist activity liked the NE, and may play a role in hypertension. In this paper, the effects of this antibody on vascular smooth muscle cell (VSMC) proliferation and its mechanism were to be studied. METHODS: The cultured rat VSMC proliferation induced by the antibodies against α1- adrenergic receptor that was purified by the immune affinity chromatography, was measured by the BrdU cell proliferation assay and cell cycle distribution. The expression of c-jun and c-fos were determined by RT-PCR and Western blotting. RESULTS: Compared to the normal IgG, the antibodies against α1-adrenergic receptor promoted the VSMC proliferation and increased the mRNA and protein expression of the c-jun significantly. The role was similar to the norepinephrine, and all was blocked by prazosin, while the mRNA and protein expression of c-fos were not affected by the antibodies. CONCLUSION: The antibodies against α1-adrenergic receptor promote the rat VSMC proliferation, and increase the expression of c-jun, which maybe play a role in the vascular remodeling in hypertension.