刺五加苷预适应对大鼠心肌缺血再灌注损伤的保护作用

目的 观察刺五加苷预适应对大鼠离体心脏缺血再灌注损伤的保护作用,并明确相应量效关系.方法 30只SD大鼠随机分为5组,每组各6只.对照组(C组)为单纯缺血再灌注组,P1、P2、P3、P4组给予不同剂量浓度(30 mg/kg、40 mg/kg、60 mg/kg和80 mg/kg)刺五加苷预适应.采用Langendorffl离体心脏灌流模型,各组均平衡灌流15 min后全心停灌25 min,再灌注30 min.P1、P2、P3、P4组在缺血再灌注前分别用含相应浓度刺五加苷的K-H灌注液灌注10 min.记录各组心功能和冠脉流量的动态变化,并对再灌注期间的心律失常进行评分.结果 平衡末5组大鼠左室舒张末压(LVEDP)、左室舒张压(LVDP)、左心室内压最大上升速率(+do/dt max)、左室舒张末压最大下降速率(-do/dt max)间差异均无统计学意义(P>0.05).再灌注30 min后,5组LVDP、+do/dt max、-do/dt max间差异均有统计学意义(P<0.05),P2、P3组与C组LVDP、+dp/dt maxx、-do/dt max间差异均有统计学意义(P<0.05).5组大鼠再灌注后各时点(5、10、15、20、30 min)冠脉流量间差异均有统计学意义(P<0.05),P2、P3组与C组各时点冠脉流量间差异均有统计学意义(P<0.01).5组大鼠心律失常评分间差异有统计学意义(P<0.05).P2、P3组与C组评分间差异有统计学意义(P<0.05).结论 刺五加苷对缺血再灌注心肌能产生预适应样保护作用--改善心功能、增加冠脉流量和抗心律失常.刺五加苷对缺血再灌注心肌保护作用呈量效关系,其中40 mg/kg和60 mg/kg优于30 mg/kg和80 mg/kg.

Protective Effect of Eleutheroside Preconditioning to Myocardial Ischemic Reperfusion Injury in Isolated Rats

Objective To investigate the protective effect of eleutherosid preconditioning to myocardial ischemic-reperfusion injury(IRI) in isolated rats and the dose-effect relationship between eleutheroside preconditioning and the myocardial protection.Methods Male rat hearts,which were isolated and perfused with Langendorff apparatus,were randomly divided into 5 groups,6 in each.The isolated hearts were subjected to 25 min ischemia and then followed by 30 min reperfusion after 15min stabilization in control group(C...