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| 一个新的小鼠腭裂候选基因cDNA序列的克隆 | |
| 引用本文： | 李鑫,金岩,晏伟,刘源,王新文.一个新的小鼠腭裂候选基因cDNA序列的克隆[J].中华医学遗传学杂志,2005,22(5):481-484. |
| 作者姓名： | 李鑫 金岩 晏伟 刘源 王新文 |
| 作者单位： | 1. 250012,山东大学口腔医院牙体牙髓科 2. 710032,西安,第四军医大学口腔医学院病理科 3. 710032,西安,第四军医大学口腔医学院病理教研室 |
| 摘 要： | 目的 克隆并筛选与小鼠腭裂发生相关候选新基因。方法 利用聚合酶链反应改良扣除杂交技术，克隆正常组与腭裂组小鼠腭突组织中差异表达的基因，经反向点杂交鉴定后挑选阳性克隆并测序及同源性分析，Northern印迹杂交检测所获的新基因mRNA的全长。结果 经大规模测序后得4个新的表达序列标签，其中1个片段全长为809bp，Northern印迹杂交结果显示为该基因的eDNA全长。结论 获得一个新的与小鼠腭裂发生相关的候选基因的cDNA全长。 |
| 关 键 词： | 小鼠 腭裂 候选基因 cDNA 基因序列 基因克隆 聚合酶链反应 杂交技术 |
| 收稿时间： | 2005-01-13 |
| 修稿时间： | 2005年1月13日 |
Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate |
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| LI Xin,JIN Yan,YAN Wei,LIU Yuan,WANG Xin-wen.Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate[J].Chinese Journal of Medical Genetics,2005,22(5):481-484. | |
| Authors: | LI Xin JIN Yan YAN Wei LIU Yuan WANG Xin-wen |
| Institution: | Department of Oral Pathology, Stomatology College, the Fourth Military Medical University, Xi'an, Shaanxi, 710032 PR China. |
| Abstract: | Objective Cloning and screening a novel candidate gene related to developing mouse cleft palate. Methods The differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases. Resuits Four novel express sequence tags were obtained, one of which spanning 809 bp was the full-length of the novel gene cDNA identified by Northern hybridization. Collusion A novel candidate gene related to mouse cleft palate was cloned. |
| Keywords: | clone cleft palate subtractive hybridization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |