树突状细胞负载肝癌相关抗原后成熟调控的研究

目的 研究树突状细胞（DC）负载肝癌相关抗原后的成熟调控。方法 用SDS－PAGE制备电泳纯化牛结核分枝杆菌热休克蛋白70，用其诱导DC的分化与成熟。结果 DC负载肝癌可溶性抗原后，10％2细胞失去了DC特征，同时其表面CD54（90．0％），CD83（78．0％），CD86（85．0％）分子表达下降；牛分枝杆菌卡介苗－热休克蛋白70（BCG HSP70）的活化有利于负载肝癌可溶性抗原后的DC维持其特异性标志，同时DC表面CD54（92．0％），CD83（90．0％），CD86（91．0％）分子表达增加，DC诱导同种异体淋巴细胞转化的能力增加，淋巴细胞增殖加快，从而促进DC成熟，增加其抗原呈递能力。结论 预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子。

Maturation regulation of dendritic cells pulsed with hepatocellular carcinoma cell soluble antigens

Objective To research the maturation regulation of dendritic cells (DCs) pulsed with hepatocellular carcinoma (HCC) cell soluble antigens. Methods BCG HSP 70 was purified by SDS-PAGE electrophoresis and its biological activity was determined with ELISA. Phenotypes of DCs pulsed with antigens or with both antigens and BCG HSP 70 were analysed with flow cytometry. MTT assay was used to estimate the proliferation of self lymphocytes and the mixed lymphocyte reaction (MLR) of BCG HSP 70 primed DCs. Results The characteristics of DCs had changed after loaded with soluble antigens of HCC. There were about 10% DCs which had lost their specific markers. The expression levels of CD54, CD83, CD86 molecules and the stimulatory ability in allogeneic MLR decreased. However, after being activated by BCG HSP 70, the DCs pulsed with antigens could keep their special markers and the expression levels of CD54, CD83, CD86 molecules increased too. The stimulatory abilities in allogeneic MLR and proliferation of self lymphocytes also improved. Conclusion This study shows that BCG HSP 70 can induce DCs pulsed with antigens maturation and improve their antigen-presenting ability, which may be a useful maturation inducer for dentritic cells.