Modulation of inflammatory markers by the ethanolic extract of Leucas aspera in adjuvant arthritis |
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Authors: | Kripa K G Chamundeeswari D Thanka J Uma Maheswara Reddy C |
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Institution: | a Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai 600117, India b Faculty of Pharmacy, Sri Ramachandra University, Porur, Chennai 600116, India c Department of Pathology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Porur, Chennai 600116, India |
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Abstract: | Aim of the studyTo investigate the anti-inflammatory and antioxidant potential of ethanolic extract of Leucas aspera (EELA) in adjuvant arthritis.Materials and methodsComplete Freund's adjuvant served to induce arthritis. EELA was administered in two doses along with vehicle control (0.1% carboxymethyl cellulose) and positive control (Diclofenac). Levels of tumour necrosis factor (TNF-)-α, C-reactive protein (CRP), Interleukin-2 (IL-2), Cathepsin D, activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were estimated in plasma/hemolysate and tissue. HPLC analysis of EELA was also performed.ResultsEELA exhibited significant anti-inflammatory (p < 0.001) and antioxidant activity (p < 0.001). It did not show mortality up to 2000 mg/kg body weight. Histopathological studies confirmed complete cartilage regeneration and near normal joint in EELA2 treated arthritic rats.ConclusionThis study highlighted the antioxidant and anti-inflammatory potential of Leucas aspera. Three major families of compounds present in EELA may explain these activities: catechins (epicatechin, beta epicatechin), flavonoids (procyanidin), phytosterols (beta-sitosterol) apart from glycosides, phenolic compounds and tannins. |
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Keywords: | Leucas aspera Anti-inflammatory Adjuvant arthritis Antioxidant Ethanolic extract |
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