G6PD缺乏时红细胞膜磷脂脂肪酸组成的改变

探讨Ｇ６ＰＤ缺乏时红细胞膜磷脂的氧化性损伤用氯仿：甲醇（２∶１Ｖ／Ｖ）抽提膜脂质，通过硅胶柱层析分离得到磷脂，经薄层层析分离得磷脂酰乙醇胺（ＰＥ）、磷脂酰丝氨酸（ＰＳ）和磷脂酰胆碱（ＰＣ）采用ＴＬＣ扫描仪测定各种磷脂的含量用甲醇提取ＰＥ，ＰＳ及ＰＣ，经快速酯交换得到脂肪酸甲酯、采用毛细管气相色谱分析各种磷脂中脂肪酸的组成情况结果：Ｇ６ＰＤ缺乏时，ＰＳ含量下降，红细胞膜总磷脂及ＰＳ中不饱和脂肪酸（Ｃ１８∶１，Ｃ１８∶２）的含量均减少，ＰＥ中Ｃ１６∶０的含量也减少实验结果推测，Ｇ６ＰＤ缺乏时红细胞膜磷脂改变，ＰＳ含量的减少及ＰＳ中不饱和脂肪酸含量的下降可能与氧化性损伤有关

Structural Change of Erythrocyte Membrane Phosphalipid in G6PD Deficiency

Studied was the oxidative damage of eythrocyte membrane phosphalipid in G6PD deficiency. With chloroform and methanol (2:1 V/V ), lipid was extracted through silica-gel column chromatography and further decomposed into PE, PS and PC, which were then measured by TLC screener. Methanol was used to extract PE, PS and PC, from which fatty acid methyl ester was got through rapid lipid exchange. With capillary collumn gas chromatography the structure of fatty acid was analyzed in the phosphalipid. Result: G6PD deficiency witnessed the decrease of PS content; the contents of unsaturated fatty acids (C 18 :1, C 18 :2) were both reduced in total phosphalipids and PS; and the content of C 16 :0 fell in PE. From the experimental consequence it might be inferred that when G6PD is short, the oxidative damage may have a bearing on the phosphalipid of eythrocyte membrane, the loss of PS content and the content drop of unsaturated fatty acid.