血清高迁移率族蛋白B1与新生儿呼吸窘迫综合征的相关性研究

目的探讨血清高迁移率族蛋白B1(HMGB1)水平与新生儿呼吸窘迫综合征(NRDS)的相关性。方法选取NRDS患儿35例(观察组)及正常新生儿35例(对照组),收集两组新生儿生后12~24 h内外周静脉血,采用酶联免疫法(ELISA)检测两组新生儿血清HMGB1水平。结果 NRDS患儿血清HMGB1水平高于对照组(P0.05),重度NRDS患儿血清HMGB1水平高于轻度患儿(P0.05),死亡的NRDS患儿血清HMGB1水平高于存活患儿(P0.05)。ROC曲线分析显示预测NRDS的AUC值为0.846(95%CI:0.755~0.936),血清HMGB1 625.3 pg/m L为预测NRDS的最佳界值,预测NRDS患儿死亡风险的AUC为0.916(95%CI:0.813~1.000),血清HMGB1 772.2 pg/L为预测NRDS患儿死亡的最佳界值。结论 NRDS患儿血清HMGB1水平显著升高,血清HMGB1可较好地预测NRDS的发生及预后。

Association between high-mobility group box 1 and neonatal respiratory distress syndrome

Objective To study the association between serum level of high-mobility group box 1(HMGB1) and neonatal respiratory distress syndrome (NRDS). Methods A total of 35 infants with NRDS and 35 normal neonates (control group) were enrolled. Peripheral venous blood samples were collected with 12-24 hours after birth. ELISA was used to measure the serum level of HMGB1. Results The infants with mild and severe NRDS had a significantly higher serum level of HMGB1 than the control group (P<0.05). The infants with severe NRDS had a significantly higher serum level of HMGB1 than those with mild NRDS (P<0.05). The infants with NRDS who died had a significantly higher serum level of HMGB1 than those who survived (P<0.05). The receiver operating characteristic (ROC) curve showed that the optimal cut-off value for serum level of HMGB1 to predict NRDS was 625.3 pg/mL with an area under the ROC curve (AUC) of 0.846 (95%CI: 0.755-0.936), and the optimal cut-off value for serum level of HMGB1 to predict the death of infants with NRDS was 772.2 pg/mL with an AUC of 0.916 (95%CI: 0.813-1.000). Conclusions Infants with NRDS have a significant increase in the serum level of HMGB1, and the serum level of HMGB1 can well predict the development and prognosis of NRDS.