宫颈鳞癌中MK蛋白的表达与肿瘤血管新生的相关性研究

目的:探讨宫颈鳞癌组织中MK蛋白的表达及其与肿瘤血管新生的关系,为宫颈鳞癌的化学防治提供实验依据.方法:采用免疫组织化学S-P法检测58例宫颈鳞癌和10例正常宫颈上皮组织中MK蛋白的表达,并对其微血管(CD34标记)计数.结果:宫颈鳞癌组织中MK蛋白的表达及微血管密度(MVD)均明显高于正常宫颈上皮组织(P＜0.01),MK蛋白的高表达与宫颈鳞癌病灶的大小及临床分期相关性显著(P=0.045;P=0.002),与患者年龄、肿瘤分化程度及淋巴结转移无明显相关;MK蛋白表达阳性者微血管密度明显高于阴性者,二者正相关(P=0.043).结论:MK蛋白过表达可能参与了宫颈鳞癌的发生发展,并与宫颈鳞癌的血管新生密切相关,可作为宫颈鳞癌化学防治的一个新靶点.

Expression of Midkine protein in cervical squamous cell carcinoma and its correlation with angiogenesis

Objective:To investigate the expression of MK protein and its correlation with neovascularization in cervical squamous cell carcinoma,so as to provide experimental foundation for prevention and therapy of CSCC. Methods:The expression of MK protein and microvascular density(MVD) labeled by CD34 were examined by immunohistochemistry S-P method in 58 cases of CSCC and 10 cases of normal cervical epithelium. Results:The expression of MK protein and MVD were significantly higher in CSCC than that in normal cervical epithelium(P < 0.01). Overexpression of MK protein in CSCC was related to tumor size and FIGO staging,but had no relation to age,differentiation degree and lymphnode metastasis. The MVD was significantly higher in the MK protein positive tumors than in negative ones(P < 0.05). Conclusion:The expression of MK protein might play an important role in carcinogenesis and development of CSCC and have close relation with tumor neovascularization. MK could be taken as a new target for prevention and therapy of CSCC.