慢性移植物抗宿主病患者T细胞受体Vβ亚家族T细胞的免疫重建

目的：了解慢性移植物抗宿主病（graft-versus-host disease,GVHD)患者T细胞受体（Tcell recepter,TCR)VβrT细胞免疫功能重建的情况。寻找可能与GGVHD相关的TCRVβ亚家族基因。方法：采用RT-PCR方法扩增5例患者外周血单个核细胞的TCRVβ24个亚家族基因，分析其TCRVβ亚家族基因。方法：采用RT-PCR方法扩增测到2-8个Vβ亚家族的T细胞，不同患者所缺乏的TCRVβ亚家族不尽相同，其中4例患者均表达Vβ基因。结论：慢性GVHD患者外周血TCRVβ亚家族T细胞部分受抑制，Vβ亚家族T细胞可能和慢性GVHD有关，此方法不仅可以更详细地了解病人T细胞免疫功能重建的情况，更重要的是今后有可能在分子水平上寻找与GVHD相关的TCRVβ亚家族基因，为临床开展特异性的GVHD细胞亚群清除性治疗提供实验依据。

Immunological Reconstitution of T Cell Receptor Repertoire Diversity in Patients with Chronic Graft-versus-host Disease

Objective： The current stady was designed to investigate the reconstitution of T cell receptor repertoire in the patients with chronic Graft-versus-host disease (cGVHD). Methods： The TCR repertoire in peripheral blood mononuclear cells from 5 cGVHD patients was examined after PCR amplification of 24 Vβ gene subfamilies. Results： Only 2－8 Vβ subfamily T cells were found in the samples from these patients, and there were different demonstrations in different patients. We found Vβ T cells proliferated in 4 patients. Conclusion： The TCR repertoire complexities was abnormal in all patients,Vβ may be associated with cGVHD, and the method may be demonstrated the reconstitution of T cell after transplantation.