| 野菊花注射液中吐温-80含量的测定 |
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| 引用本文： | 张俏,史文静,曹爱兰.野菊花注射液中吐温-80含量的测定[J].亚太传统医药,2012,8(6):39-40. |
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| 作者姓名： | 张俏 史文静 曹爱兰 |
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| 作者单位： | 陕西中药研究所,陕西,咸阳,712000 |
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| 摘 要： | 目的:建立野菊花注射液中吐温-80的含量测定方法。方法:采用紫外分光光度法,经硫氰酸钴铵溶液显色,测定野菊花注射液中吐温-80含量。结果:吐温-80在波长625nm处有最大吸收,在1.1292～3.3876mg/mL范围内呈良好线形关系(n=5),回归方程Y=0.1688X+9.52×10-2,r=0.9996,平均回收率为101.2%,RSD=0.96%。结论:该方法灵敏度高,稳定可靠,重现性好,适用于野菊花注射液中吐温-80含量的测定。
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| 关 键 词： | 野菊花注射液 吐温-80 紫外分光光度法 含量测定 |
UV-spectrophotometry Determination of Tween 80 in Yejuhua Injection |
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| Zhang Qiao
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Shi Wenjing
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Cao Ailan.UV-spectrophotometry Determination of Tween 80 in Yejuhua Injection[J].Asia-Pacific Traditional Medicine,2012,8(6):39-40. |
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| Authors: | Zhang Qiao Shi Wenjing Cao Ailan |
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| Institution: | (Shaanxi institute of traditional Chinese medicine, Shanxi 712000,China) |
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| Abstract: | Objective：set up a content determination method for Tween 80 which within Yejuhua injection. Methods：according to UV-spectrophotometry and cobaltous thiocyanate coloration, determining the actual Tween 80 content in Yejuhua injection. Results： about Tween 80, there is a maximum absorption at a wavelength of 625nm ; a wonderful liner relationship exist range from 1. 1292 to 3. 3876mg （n=5）, the regression equation as follow： Y： 0. 1688X＋9.52 ×10^-2 ,r= 0. 9996; the average recovery is 101. 2% , RSD= 0.96 %. Conclusion：the Tween 80 content determination method is sensitive, stable and reliable and also with good reproducible. The method is suitable for Tween 80 content deternination of the Yejuhua injection. |
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| Keywords: | Yejuhua Injection Tween 80 UV spectrophotometry Content Determination |
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