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Adipose tissue derived mesenchymal stem cell (AD-MSC) promotes skin wound healing in diabetic rats
Authors:Maharlooei Mohsen Khosravi  Bagheri Mansooreh  Solhjou Zhabiz  Jahromi Behnam Moein  Akrami Majid  Rohani Lili  Monabati Ahmad  Noorafshan Ali  Omrani Gholamhossein Ranjbar
Affiliation:a Cell and Molecular Medicine Research Group, Student Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
b Resident of General Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran
c Laboratory for Stem Cell Research, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran
d Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran
e Histomorphometry & Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
f Endocrine and Metabolism Research Centre, Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:

Aims

Stem cells are a new hope to ameliorate impaired diabetic wound healing. The purpose of this study was to evaluate the effect of adipose tissue derived mesenchymal stem cells (AD-MSCs) on wound healing in a diabetic rat model.

Methods

Twenty-six rats became diabetic by a single intraperitoneal injection of streptozotocin. Six rats served as non-diabetic (non-DM). Diabetic rats were divided into two equal groups randomly; control and treatment. Six weeks later, a full-thickness circular excisional wound was created on the dorsum of each rat. AD-MSCs were injected intra-dermally around the wounds of treatment group. PBS was applied to control and non-DM groups. The wound area was measured every other day. After wound healing completion, full thickness skin samples were taken from the wound sites for evaluation of volume density of collagen fibers, length and volume density of vessels, and numerical density of fibroblasts by stereological methods.

Results

AD-MSCs accelerated wound healing rate in diabetic rats, but did not increase length and volume density of the vessels and volume density of the collagen fibers. AD-MSCs decreased the numerical density of fibroblasts.

Conclusions

We concluded that AD-MSCs enhances diabetic wound healing rate probably by other mechanisms rather than enhancing angiogenesis or accumulating collagen fibers.
Keywords:AD-MSC   Diabetes   Wound healing
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