生物血管基质材料的抗原表达及力学特性

了解胰蛋白酶处理前后版纳微型猪近交系血管的抗原表达及力学特性，为异种移植及猪血管用于血管组织工程基质材料提供资料，取猪颈动脉，胰蛋白酶预处理猪血管前后不同时相点取标本石蜡包埋，采用组织形态学方法结合计算机图像分析，对猪血几何形态及纤维结构进行形态定量学研究，分别测定胰蛋白酶作用前后猪动脉压力直径关系，冰冻切片，用亲和免疫组化法检测α-gal抗原表达。结果表明α-gal抗原仅表达于血管内皮细胞，胰蛋白酶预处理血管后，未见α-agl抗原表达，血管内细胞包括内皮细胞，平滑肌细胞已去除，HE染色未见纤维断裂，血管无明显形变，处理前后血管顺应性相差不显著，提示经胰蛋白酶预处理的猪血管抗原性大大降低，其力学特性相差不显著，可用于血管基质材料。

Antigen Expression and the Biomechanical Characteristics of the Biologic Blood Vessel Matrix

To explore the changes of the antigen expression and the biomechanical characteristics of blood vessel in Banna little ear pig before and after trypsin treatment, and provide data for xenotransplantation and pig vessel using for tissue engineering. Geometric morphology and microstructure of pig cartoid artery were stuided quantitatively by histologic method and computer image analysis. The relationship between pressure and diameter was observed at different period of time before and after trypsin treatment. Affinity-immunohistochemistry assay was conducted to detect the expression of xenoantigens (alpha-Gal). The results showed that alpha-Gal antigen is only expressed in vascular endothelial cellsouly. There is no significant difference in blood vessel compliance. These demonstrate that the antigenicity of pig carotid artery is significantly reduced, however, the mechanical characteristics did not change significantly. We suppose that pig vessels treated by trypsin can be used as the substrate material for vascular tissue engineering.