参知健脑方组分对淀粉样β蛋白β25-35诱导的SH-SY5Y细胞凋亡的保护作用

目的:探讨SZJ对淀粉样β蛋白25-35(Aβ25-35)诱导的SH-SY5Y细胞凋亡的保护作用机制。方法:以Aβ25-35诱导SH-SY5Y细胞凋亡,采用MTT比色分析测定细胞存活率,应用Western blot检测Aβ25-35以及SZJ/APP17肽对SH-SY5Y细胞Bax、Bcl-2、Caspase-3表达的影响。结果:25μmol/L的Aβ25-35减低SH-SY5Y细胞存活率,SZJ/APP17肽预处理24h可提高SH-SY5Y细胞存活率,相同浓度Aβ25-35 SZJ预处理组与非处理组比较差异显著(P<0.05);25μmol/L Aβ25-35处理的SH-SY5Y细胞Bax表达增加,Bcl-2表达减少,Caspase-3的表达增高,SZJ/APP17肽预处理能抑制其表达升高,与非预处理组比较差异显著(P<0.05)。结论:SZJ可对抗Aβ25-35对SH-SY5Y细胞的毒性作用,其机制可能与抑制Bax、Caspase-3的表达,促进Bcl-2的表达有关。

Protective Effects of SZJ against SH-SY5Y Cell Apoptosis Induced by β-amyloid Peptide 25-35

Objective: To investigate the mechanism of the protective effects of SZJ against apoptosis of SH-SY5Y cells induced byβ-amyloid 25-35 and SZJ/APP17 peptide were assessed by Western blot.Methods:Based on the model of apoptosis of SH-SY5Y cells induced by Aβ25-35,cell viability was determined by MTT assay.The expressions of Bax,Bcl-2,Caspase-3 of SH-SY5Y cells induced by Aβμmol/L Aβ25-35.Results:25 peptide25-35(Aβ25-35)lowered the cell viability of SH-SY5Y cells,SZJ pretreatment enhanced the cell viability of SH-SY5Y cells,and there was significant difference compared with SZJ non-pretreated groups(P<0.05).25μmol/L Aβ25-35 treatment up-regulated the Bax expression of SH-SY5Y cells,down-regulated the Bcl-2 expression and SZJ pretreatment down-regulated Bax expression and up-regulated Bcl-2 expression,and difference was significant compared with SZJ non-pretreated groups(P<0.05).25μmol/L Aβ25-35 treatment up-regulated the Caspase-3 expression of SH-SY5Y cells,25μmol/L SZJ pretreatment down-regulated its expression,the difference was significant compared with SZJ non-pretreated groups(P<0.05).Conclusion:SZJ can antagonize Aβ25-35 neurotoxicity to the SH-SY5Y cells.The mechanism of protection is likely related to a lowering in the Bax and Caspase-3 expression and a rasing in the Bcl-2 expression.