睾丸Sertoli细胞对共移植同种异体肾细胞的保护作用及受体性别的影响

目的 探讨表达FasL的睾丸Sertoli细胞对不同性别受体在鼠中移植肾细胞的保护作用，方法 用酶消化法制备Sertoli细胞及肾细胞，流式细胞仪检测细胞表面FasL及Fas的表达，将约10^6个细胞（注入的细胞因分组不同而异，见1.2.3)注入异体肾包膜下，用SABC法测定肾细胞的存活状况，并以TUNEL法观察移植物中的淋巴细胞凋亡。结果 移植后20d，用组织学分析移植细胞的存活率，单纯肾细胞移植，移植物无一例存活；混合细胞移植，移植物的存活率为87.5%。以抗FasL抗体处理过的Sertoli细胞与肾细胞共移植，肾细胞的存活率仅为30.0%，雌性受体移植物的存活率为77.8%，移植物中可见凋亡的淋巴细胞，结论 同种异体移植中，Sertoli细胞对移植的肾细胞具有保护作用，其机制与Fas/FasL系统介导的淋巴细胞凋亡有关。

The protective effect onthe co-transplanted allogeneic renal cells in male and female recipient rats by thetesticular Sertoli cells expressing FasL

Aim To explore the protective effect of Sertoli cells on the co-transplanted allogeneic renal cells in male and female recipient rats. Methods Testicular Sertoli cells were prepared by digestion with trypsin， collagenase and DNase， while renal cells were prepared by digestion with trypsin alone. FasL and Fas expressions were detected respectively by FCM before transplantation. About 106 cells were injected into the allogeneic renal subcapsule. In order to demonstrate the survival of renal cells， the expression of g-PG in graft was examined by SABC method. Apoptosis of the lymphocytes surrounding the graft was observed by TUNEL(terminal deoxynucleotidyl transferase-mediated X-dUTP nick end labeling). Results The grafts were analyzed histologically 20 days after transplantation. The renal cells transplanted alone were all rejected, while the survival rates of the mixed cell transplantation were 87.5％ and 77.8％ in male and female recipients respectively. When the renal cells co-transplanted with Sertoli cells treated by anti-FasL mAb， only 30.0％ grafts were survived. Apoptosis of lymphocytes surrounding the graft were quite evident. Conclusion Sertoli cells expressing FasL can protect renal cells from allograft rejection by inducing apoptosis of Fas expressing T cells surrounding the grafts.