PD-ECGF及Pan caspase-1在胃腺癌组织中的表达及临床意义

目的研究血小板衍生内皮细胞生长因子（PD-ECGF）及Pan caspase-1在胃腺癌组织中的表达,进一步探讨PD-ECGF在胃腺癌血管形成中的作用及Pan caspase-1对胃腺癌细胞凋亡基因蛋白的影响,为临床正确判断肿瘤细胞的生物学行为及估计患者预后、筛选可靠生物学指标。方法收集2001-2003年本院胃腺癌手术标本蜡块54例。其中低分化胃腺癌32例,高分化22例。采用SP免疫组化法,严格按照SP超敏试剂盒的说明操作,显微镜下观察PD-ECGF和Pan caspase-1在胞浆/胞核中的表达。并结合F-ⅧR Ag检测,测量MVD值。结果PD-ECGF在低分化腺癌表达为75.0%,高分化表达为36.4%,两者有显著性差异（P〈0.05）。Pan caspase-1在低分化腺癌表达为21.9%,高分化表达为72.8%,两者有显著性差异（P〈0.01）。肿瘤的MVD在PD-ECGF和Pan caspase-1的阳性表达中分别高于其阴性表达（P值分别是0.0033和0.0149）。结论PD-ECGF有助于胃腺癌间质血管形成,与癌组织分化程度有关。Pan caspase-1在癌细胞凋亡有作用,与癌组织分化程度有关。提示联合检测PD-ECGF和Pan caspase-1有助于确定癌细胞的分化程度,正确判断胃癌细胞的生物学行为及患者预后。

Expressions of PD-ECGF and Pan Caspase-1 in Human Gastric Adenocarcinoma Tissue and Their Clinical Significance

Objective To investigate the relationship between tumor angiogenesis and the expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase(PD-ECGF/dThdPase).Although it is reported that Pan caspase-1 protein was involved in apoptosis,apoptosis effects mutually generated MVD and Pan caspase-1 have not yet been evaluated. Methods The expressions of PD-ECGF and Pan caspase-1 in 54 gastric adenocarcinoma tissue samples,including 32 low differentiated gastric adenocarcinoma and 22 well differentiated gastric adenocarcinoma were assessed immunohistochemically,and the microvessel density(MVD) was determined by immunostaining for factor VIII-related antigen. Results Out of the 32 low differentiated gastric adenocarcinoma tissue samples, 24(75.0%) were PD-ECGF positive,and 7(21.9%) were Pan caspase-1-positive;out of the 22 well differentiated gastric adenocarcinoma tissue samples,8(36.4%) were PD-ECGF-positive,16(72.8%) were evaluated as Pan caspase-1-positive.The expressions of PD-ECGF and Pan caspase-1 were significantly associated with degrees of differentiated gastric carcinoma(P<0.05,P<0.01).The microvessel density was significantly higher in PD-ECGF-and Pan caspase-1-positive tumors than that in PD-ECGF-and Pan caspase-1-negative tumors(P=0.033,0.0149). Conclusions The results suggest that expression of PD-ECGF plays a role in the promotion of angiogenesis in human gastric adenocarcinoma and MVD has effect on expression of Pan caspase-1,which induces apoptosis in human gastric adenocarcinoma.