活性炭纳米粒子对人胃癌BGC-823细胞作用的体外实验研究

目的：探讨活性炭纳米粒子（activated carbon nanoparticles，ACNP）对人胃癌BGC-823细胞的作用。方法：将ACNP以不同浓度和时间作用于BGC-823细胞，通过MTT试验观察ACNP对细胞增殖的抑制作用；测定培养上清液乳酸脱氢酶（LDH）活性，判断细胞损伤状况；用甲基绿-派洛宁染色法及透射电镜观察细胞凋亡形态；用流式细胞术检测细胞凋亡率和细胞周期。结果：ACNP能明显抑制BGC-823细胞的增殖，呈剂量和时间依赖性，作用24，48，72h后的半数抑制浓度（IC50）分别是1．57，1．18，0．87g／L。低浓度ACNP（0．1，0．2g／L）作用24h后，LDH漏出量与对照组相比没有显著差异。0．1g／L的ACNP作用24h后，细胞出现凋亡特征的形态学改变。0．1，0．2g／L的ACNP作用24h后，细胞凋亡率分别为（5．01±1．16）％、（8．21±1．63）％，与对照组（2．48±0．58）％比较均有显著性差异；ACNP作用组的S期细胞所占比例明显增高。结论：ACNP抑制BGC-823细胞的增殖，可使细胞阻滞于S期。诱导细胞凋亡。

Effects of activated carbon nanoparticles on human gastric carcinoma cell line BGC-823 in vitro

Objective ：To investigate the effect of the activated carbon nanoparticles（ACNP） on the human gastric carcinoma cell line BGC-823 in vitro. Methods： BGC-823 cells were treated with ACNP at various concentrations for different durations. The inhibitory effect of ACNP on the proliferation of BGC-823 cells was observed by MTr assay. The lactate dehydrogenase（LDH） leakages in the culture medium were determined with LDH activity detection kit. The apoptosis-related alterations in morphology were observed by methyl green-pyronin staining and transmission electron microscopy（TEM）. The apoptotic rate and the cell cycle of BGC-823 cells were examined by flow cytometry（FCM）. Results： ACNP significantly inhibited the proliferation of BGC-823 cells in a dose- and time-dependent manner. The 50% inhibitory concentrations （ICso） after 24, 48 and 72 hours were 1. 571 1.18 and 0. 87 g/L,respectively. The LDH leakages at concentrations of 0.1 g/L and 0.2 g/L were not higher than that of the control group at 24 hours. The cells treated with 0.1 g/L ACNP for 24 hours exhibited characteristics of apoptosis. The apoptotic rates at concentrations of O. 1 g/L and 0.2 g/L were（5. 01±1.16） % and （8.21 ± 1.63 ） %, respectively, both of which were significantly higher than that of the control group （2.48±0.58 ） % at 24 hours. In ACNP group, the percentage of cells at S phase was higher than that in control group（ P 〈 0.01 ）.Conclusion： The ACNP could inhibit proliferation, cause S-phase arrest and induce apoptosis of BGC-823 cells in vitro.