| 薄层扫描法测定复方银花口服液中绿原酸的含量 |
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| 引用本文： | 聂松柳,孟楣. 薄层扫描法测定复方银花口服液中绿原酸的含量[J]. 安徽医药, 2010, 14(10): 1147-1149. DOI: 10.3969/j.issn.1009-6469.2010.10.012 |
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| 作者姓名： | 聂松柳 孟楣 |
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| 作者单位： | 安徽省六安市人民医院药剂科,安徽,六安,237005;安徽中医学院第一附属医院药学部国家中医药管理局中药制剂三级实验室,安徽,合肥,230031 |
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| 摘 要： | 目的建立复方银花口服液中绿原酸的含量测定方法。方法薄层扫描法。展开剂：醋酸丁酯-甲酸-水（7∶2.5∶2.5）的上层溶液,检测波长λS=330 nm,λR=650 nm。结果绿原酸在0.240～1.440μg范围内呈良好线性关系,r=0.99626,平均回收率=98.4%,RSD=2.34%。结论本法简单,准确,重现性好,可用于复方银花口服液中绿原酸的含量测定。
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| 关 键 词： | 薄层扫描法 绿原酸 复方银花口服液 含量测定 |
Determination of chlorogenic acid in fufangyinhua oral fluid by TLC-scanning |
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| NIE Song-Liu,MENG Mei. Determination of chlorogenic acid in fufangyinhua oral fluid by TLC-scanning[J]. Anhui Medical and Pharmaceutical Journal, 2010, 14(10): 1147-1149. DOI: 10.3969/j.issn.1009-6469.2010.10.012 |
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| Authors: | NIE Song-Liu MENG Mei |
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| Affiliation: | NIE Song-Liu1,MENG Mei2(1.The Luan People's Hospital of TCM,Luan,Anhui 237005,2.The First Affiliated Hospital of Anhui College of TCM,State Administration of National Chinese Drugs Medicament Laboratory of grade 3,Hefei,Anhui 230031) |
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| Abstract: | Aim To establish a method for determination of chlorogenic acid in fufangyinhua oral fluid.Method Chlorogenic acid in fufangyinhua oral fluid was determinated by TLC-scanning with a mixture of upper solution n-butyl acetate-methanoic-water(7∶2.5∶2.5) as the developing system.The detection wavelength was 330 nm and 650 nm.Results The calibration curve was linear in the range of 0.240 ～ 1.440 μg,r = 0.99626,average recovery rate was 98.4%,RSD = 2.34%.Conclusion The method was simple,accurate,reproducible and suitable for the content determination of chlorogenic acid in fufangyinhua oral fluid. |
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| Keywords: | TLC-scanning chlorogenic acid fufangyinhua oral fluid content determination |
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