| 致血液系统感染大肠埃希菌中Ⅰ类整合子参与耐药的研究 |
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| 引用本文： | 白松涛,兰全学.致血液系统感染大肠埃希菌中Ⅰ类整合子参与耐药的研究[J].现代预防医学,2006,33(8):1346-1347. |
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| 作者姓名： | 白松涛 兰全学 |
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| 作者单位： | 1. 深圳市龙岗区血站,518116
2. 深圳市疾病预防控制中心 |
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| 摘 要： | 目的：探讨致血液系统感染大肠埃希菌中Ⅰ类整合子介导耐药性的分子机制。方法：应用全自动细菌分析仪Microscan WalkAway40和纸片扩散法，对16株大肠埃希菌进行抗生素敏感性测定，PCR扩增细菌总DNA上的Ⅰ类整合子，对扩增产物测序并分析其中的基因盒。结果：8株细菌含有Ⅰ类整合子，整合子大小分别为600bp、1700bp和2500bp，600bp整合子含基因盒dfr2d，1700bp整合子含基因盒dfr17-aadm5。结论：整合子在介导细菌耐药性方面发挥着重要作用。
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| 关 键 词： | 大肠埃希菌 整合子 基因盒 耐药 |
| 文章编号： | 1003-8507（2006）08-1346-02 |
| 收稿时间： | 2006-04-27 |
| 修稿时间： | 2006年4月27日 |
THE STUDY ON CLASS 1 INTEGRONS IN MEDIATION OF ANTIBIOTIC RESISTANCE IN ISOLATES OF ESCHERICHIA COLI FROM BLOOD INFECTION |
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| BAI Song-tao,LIU Quan-xue.THE STUDY ON CLASS 1 INTEGRONS IN MEDIATION OF ANTIBIOTIC RESISTANCE IN ISOLATES OF ESCHERICHIA COLI FROM BLOOD INFECTION[J].Modern Preventive Medicine,2006,33(8):1346-1347. |
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| Authors: | BAI Song-tao LIU Quan-xue |
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| Institution: | LongGang Blood Station of ShenZhen, 518116 |
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| Abstract: | Objective:To explore the molecular mechanism of class 1 integrons in mediation of antibiotic resistance in isolates of Escherichia coli from blood infection.Methods:The antimicrobial susceptibility test of 16 Escherichia coli isolates were performed by Microscan WalkAway40 and the disk diffusion assay.Class 1 integrons were detected by PCR with total DNA.The purified amplicons of class 1 integrons were sequenced,and gene cassettes were identified.Results:8 isolates carried class 1 integrons.The sizes of class 1 integrons were 600bp,1700bp and 2500bp respectively.600bp integron carried gene cassette dfr2d,and 1700bp integron carried gene cassette dfr17-aadA5.Conclusion:Integrons play an important role in mediation of antibiotic resistance. |
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| Keywords: | Escherichia coli Integrons Gene cassettes Antibiotic resistance |
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