芍药苷对培养小鼠皮层神经元的保护作用

目的　探讨芍药苷是否促进培养皮层神经细胞的存活 ,并对抗兴奋性氨基酸海人藻酸 (KA)所致的神经损伤。方法　解剖分离 15d胚胎小鼠皮层神经细胞 ,接种于 2 4孔板中加药培养 ,台盼蓝染色细胞 ,相差显微镜及免疫组织细胞化学方法进行形态学观察。结果　①加入芍药苷 2 0 .8和 4 1.6mg·L- 1培养 4d增加神经细胞存活数量 ,降低死亡率。②加入KA 5 0 μmol·L- 1作用 30min ,神经元肿胀 ,失去折光性 ,核偏位 ,死亡率增高。预先加入芍药苷2 0 .8和 4 1.6mg·L- 1培养 4d ,可对抗KA所致的神经损伤。结论　芍药苷可增加神经细胞存活数量 ,降低死亡率 ,对抗KA所致的兴奋性神经损伤。

Protective effects of paeoniflorin on cultured cortical neurons of mice

AIM To determine whether paeoniflorin (PF) can increase the survival neurons and provide protection against the excitotoxic effect of kainic acid (KA) on primary cortical cultures. METHODS ]Primary cultured neurons were dissociated from 15 d old mouse embryo and plated on 24-well culture- plates with PF in the medium, neurons in the experimental group were treated with KA(50 μmol·L^-1) on d 3. Dying cells were identified by trypan blue, phase contrast microscope and immunocytochemistry were used to observe the morphology of the neurons. RESULTS ①Culturing cortical neurons with PF at 20.8 and 41.6 mg·L^-1 for 4 d increase the survival of neurons and decreased the death rate of cells. ②Damaged neurons induced by cultured with KA (50 μmol·L^-1) for 30 min showed various changes, such as cell swelling, lacking of dioptre, nucleus shifting to the cell periphery, and higher mortality. While pretreated with PF 20.8 and 41.6 mg·L^-1 for 4 d, the cortical neurons were resistant to the injury of KA. CONCLUSION PF can increase the mouse cortical neuronal survival rate, decrease the mortality and protect neurons against KA induced neurotoxicity.