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| 神经生长因子在豚鼠耳蜗中的分布及其意义 | |
| 引用本文： | 池君,张学渊,宋武战,石东文. 神经生长因子在豚鼠耳蜗中的分布及其意义[J]. 听力学及言语疾病杂志, 2006, 14(6): 445-446,448,I0002 |
| 作者姓名： | 池君 张学渊 宋武战 石东文 |
| 作者单位： | 1. 第三军医大学附属西南医院耳鼻咽喉科,重庆,400038 2. 成都军区昆明总医院 3. 疾病蛋白质组学重庆市市级重点实验室 |
| 摘 要： | 目的观察神经生长因子(nerve growthfactor,NGF)在正常豚鼠耳蜗中的定位分布,为进一步研究其在听觉通路中的作用机制提供依据。方法健康杂色豚鼠20只,制备耳蜗石蜡切片,用兔抗小鼠神经生长因子多克隆抗体行免疫组织化学染色(SP法),检测NGF在耳蜗中的表达。结果在正常豚鼠耳蜗中,NGF在螺旋神经节细胞、内外毛细胞、支持细胞、听觉神经纤维中均有表达,细胞内定位主要在胞浆。结论NGF在听觉感受器和听觉通路中有分布,表明在听觉功能的维持中起着一定的作用。 |
| 关 键 词： | 神经生长因子(NGF) 耳蜗 免疫组织化学 |
| 文章编号： | 1006-7299（2006）06-0445-03 |
| 收稿时间： | 2006-04-28 |
| 修稿时间： | 2006-04-28 |
Expression and Significance of NGF in the Cochlea of Guinea Pigs |
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| Chi Jun, Zhang Xueyuan, Song Wuzhan,et al.. Expression and Significance of NGF in the Cochlea of Guinea Pigs[J]. Journal of Audiology and Speech Pathology, 2006, 14(6): 445-446,448,I0002 | |
| Authors: | Chi Jun Zhang Xueyuan Song Wuzhan et al. |
| Affiliation: | Chi Jun, Zhang Xueyuan, Song Wuzhan, et al. |
| Abstract: | Objective To investigate the distribution of NGF in the cochleae of guinea pigs and to explore the mechanism of NGF in the auditory path ways.Methods Twenty healthy guinea pigs were selected and their cochlear paraffin sections were immunolabeled with a rabbit anti-NGF polyclonal antibody by SP method.Results NGF immunoexpression was observed in inner and outer hair cell bodies,supporting cell bodies and nerve fibers throughout the cochlea and in spiral ganglion neurons. The distribution was mainly located in the cytoplasm.Conclusion The distribution of NGF is closely related with auditory maintaince. |
| Keywords: | Nerve growth factor(NGF) Cochlea Immunohistochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |