| 正交实验法优选枇杷叶药渣中乌索酸提取工艺 |
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| 引用本文： | 康艳萍,肖道安,邹盛勤,陈武.正交实验法优选枇杷叶药渣中乌索酸提取工艺[J].时珍国医国药,2006,17(12):2385-2386. |
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| 作者姓名： | 康艳萍 肖道安 邹盛勤 陈武 |
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| 作者单位： | 1. 宜春学院化学与生物工程学院,江西,宜春,336000
2. 江西省天然药物活性成分研究重点实验室,宜春学院生物工程研究所,江西,宜春,336000 |
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| 基金项目： | 国家“863”计划重点资助项目(No.2002AA2Z3217) |
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| 摘 要： | 目的确定枇杷叶药渣中乌索酸的最佳超声提取工艺条件。方法采用正交设计L9(34)方法,考察乙醇浓度、料液比、超声时间和提取次数对乌索酸提取率的影响,用高效液相色谱法测定含量,并与常规提取法进行了对比。结果所考察的因素对枇杷叶药渣中乌索酸提取的影响程度为:乙醇浓度>提取次数>料液比>超声时间;超声提取的最佳条件为:A3B1C1D2。结论与常规的提取方法相比,超声提取具有提取时间短、操作简单、提取率高、无需加热等优点。
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| 关 键 词： | 枇杷叶 药渣 正交设计 乌索酸 超声提取 高效液相色谱 |
| 文章编号： | 1008-0805（2006）12-2385-02 |
| 收稿时间： | 2006-08-07 |
| 修稿时间： | 2006-09-16 |
Optimum Extraction of Ursolic Acid in the Residues of Eriobotrya japonica by Orthogonal Test |
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| KANG Yan-ping,XIAO Dao-an,ZOU Sheng-qin,CHEN Wu.Optimum Extraction of Ursolic Acid in the Residues of Eriobotrya japonica by Orthogonal Test[J].Lishizhen Medicine and Materia Medica Research,2006,17(12):2385-2386. |
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| Authors: | KANG Yan-ping XIAO Dao-an ZOU Sheng-qin CHEN Wu |
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| Institution: | 1. College of Chemistry and Bioengineering of Yichun University, Yichun 336000, China ;2. Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Bioengineering Research Institute of Yichun University, Yichun ,336000, China |
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| Abstract: | Objective To optimize the ultrasonic extraction for ursolic acid from the residues of Eriobotrya japonica. Methods According to the orthogonal design L_9(3~4),the optimum ultrasonic extraction technology was sought through experimental investigation,and was compared with the traditional extraction technology.The content of ursolic acid was determined by RP-HPLC.Results The order of factors to affect the ursolic acid extraction was A_3B_1C_1D_2.Conclusion Compared with traditional extraction,ultrasonic method can save time,is easy to operate,can improve extraction rates and needs no heating. |
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| Keywords: | Eriobotrya japonica(Thunb ) Lindl Residues Orthogonal test Ursolic acid Ultrasonic extraction HPLC |
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