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| 灯盏花素对顺铂所致豚鼠耳蜗损伤的拮抗作用 | |
| 引用本文： | 洪丽,赖晃文,熊敏.灯盏花素对顺铂所致豚鼠耳蜗损伤的拮抗作用[J].听力学及言语疾病杂志,2011,19(4):345-348. |
| 作者姓名： | 洪丽 赖晃文 熊敏 |
| 作者单位： | 1. 湖北省咸宁学院附属第二医院耳鼻咽喉科,咸宁,437100 2. 广州军区广州总医院医学实验科 3. 广州军区广州总医院耳鼻咽喉科 |
| 摘 要： | 目的探讨灯盏花素对顺铂致豚鼠耳毒性的拮抗作用。方法将30只豚鼠分为三组:正常对照组(A组)、实验对照组(B组)、实验组(C组),每组10只。B组和C组一次性腹腔注顺铂10 mg/kg,同时C组连续10天腹腔注射灯盏花素15 mg.kg-1.d-1,B组等时程腹腔注等量生理盐水。每组豚鼠在实验前后均行听性脑干反应(ABR)检测。在豚鼠顺铂致耳毒性模型完成并检测ABR反应阈后,用免疫组织化学方法检测4-羟基-2-壬烯醛(4-HNE)在各组动物耳蜗的表达,同时用扫描电镜观察各组豚鼠耳蜗形态。结果实验前三组豚鼠ABR反应阈差异无统计学意义(P>0.05),实验后B组和C组豚鼠ABR反应阈分别为50.12±18.45、36.32±15.63 dB SPL,均明显高于实验前,且B组显著高于C组(P<0.05),B组豚鼠听功能损伤明显重于C组。4-HNE在A组表达呈阴性,在B组和C组耳蜗表达呈阳性,且在B组的表达明显强于C组。B组耳蜗外毛细胞的损伤明显较C组重。结论 4-HNE在顺铂所致豚鼠耳蜗损伤中呈阳性表达。灯盏花素对4-HNE的形成有明显抑制作用,且能拮抗顺铂对豚鼠的耳蜗毒性,表明活性氧(ROS)在顺铂所致豚鼠耳蜗损伤中起重要作用。 |
| 关 键 词： | 顺铂 耳蜗 活性氧 灯盏花素 |
The Anti-Toxic Effect of Breviscapine on Cisplatin Induced Cochlea Damage in Guinea Pig |
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| Hong Li,Lai Huangwen,Xiong Min.The Anti-Toxic Effect of Breviscapine on Cisplatin Induced Cochlea Damage in Guinea Pig[J].Journal of Audiology and Speech Pathology,2011,19(4):345-348. | |
| Authors: | Hong Li Lai Huangwen Xiong Min |
| Institution: | Hong Li,Lai Huangwen,Xiong Min(The Second Hospital Affiliated to Xianning University,Xianning,437100,China) |
| Abstract: | Objective To investigate the antitoxic effect of breviscapine on cisplatin induced cochlea damage in guinea pig.Methods The experiment consisted of three groups : normal control group(group A),experimental control group(group B) and experimental group(group C) with 10 guinea pigs in every group.Cisplatin was administrated intraperitoneally to animals in group B and C for a single injection at the dose of 10 mg/kg.While cisplatin was delivered intraperitoneally to animals in group C for continuous 10 days,an... |
| Keywords: | Cisplatin Cochlea Reactive oxygen species Breviscapine |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |