Insulin-like molecules in Momordica charantia seeds |
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| Authors: | T B Ng C M Wong W W Li H W Yeung |
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| Affiliation: | 1. Vaccine Research Institute of Sun Yat-sen University, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;2. Institute of Molecular Medicine, Health Science Centre, Shenzhen University, Shenzhen 518060, China;3. Guangdong Provincial Key Laboratory of Liver Disease Research, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China;1. School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia;2. Vector Control Research Unit, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia |
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| Abstract: | Decorticated Momordica charantia seeds were extracted and processed by a method which was developed originally for the purification of insect and annelid insulins. Essentially, the method entailed HCl--ethanol extraction, neutralization with NH4OH, gel filtration on Sephadex G-50, ion exchange chromatography on CM Sepharose CL-6B and desalting on Sephadex G-10. Of the seven fractions collected, three fractions were obtained with antilipolytic and lipogenic activities in isolated adipocytes and one fraction with only lipogenic activity. The data indicate that molecules with insulin-like bioactivity are present in Momordica charantia seeds. |
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