A comparison of female and male rats' ETOH-induced ataxia and exploration following restraint or swim stress

Animal models of stress reactivity are often employed in developing treatments for humans. Many studies use shock stress, and most use male rats. These experiments compare female and male rats exposed to either restraint stress (RS) or ambient-temperature swim stress (SS), using two durations of each stressor and naive controls. The ataxic effects of a 0.6 g/kg i.p. dose of ethanol (ETOH) were measured. Females exhibited less ataxia than males following ETOH administration. There were no significant effects of stress on ETOH-induced ataxia. Exploration was also measured in an open-field test (OFT) both pre- and poststress. In the prestress OFT, females were more active than males. For the no-stress groups and the shorter-duration stress groups, exploration decreased between the first and second OFTs. However, the groups exposed to the longer-duration stress did not show this expected decrease in exploration. A key finding of this research is that while sex differences may be present at baseline, the sexes may react similarly to stress. These data extend knowledge on sex differences in stress, alcohol reactivity and exploratory behavior.