以腺嘌呤对慢性肾功能衰竭尿毒症期大鼠造模的方法研究

目的：优选腺嘌呤致大鼠慢性肾功能衰竭（CRF）尿毒症期的实验方法。方法：采用以下两种不同方法造模，造模A组给予2．5％腺嘌呤混悬液，按250mg／kg／d灌胃，连续给药14d，之后改为125mg／kg／d。造模B组给予2．5％腺嘌呤混液，按250mg／kg／d灌胃，第1-14天，每日给药1次，第14天后隔日给药，观察腺嘌呤对各种肾功能指标的影响及肾组织形态学的变化。结果：模型A组、模型B组大鼠血清BUN、SCr、uA浓度等指标均明显上升，模型B组较模型A组高；光镜下观察病理切片显示肾皮质、髓质有不同程度损伤，模型B组损伤程度较重。生化指标及形态组织学观察提示腺嘌呤CRF大鼠模型造模成功，模型B组模型肾皮质、髓质功能中重度损害、酸中毒，符合慢性肾功能衰竭中晚期尿毒症常见生化指标改变。结论：采用2．5％腺嘌呤混悬液，按250mg／kg／d连续灌胃14d后，再隔日给药14d的造模方法较佳。

Research on Modeling Method of Chronic Renal Failure Rats with Uremia Caused by Adenine

Objective： To choose the experimental technique of makingehronic renal failure（CRF） model rats with uremia caused by adenine. Methods： Two different methods were used to make models： model group A were given gastric perfusion of 2.5% adenine suspensions （250 mg/kg/d） for 14 days; then the perfusion changes to 125 mg/kg/d 14 days later. Model group B were given gastric perfusion of 2.5% adenine suspensions （250 mg/kg/d）, once a day for 14 days; 14th day later, the medicine were given every other day. Then the influence of adenine on the renal function targets and the change of kidney tectology were observed. Results： The blood serum BUN, SCr, UA density of model group A and model group B increased obviously, model group B had a high increase than model group A. Under the light microscope, pathological section demonstrated that the renal cortex and renal medulla have the damage of various degree, model group B had a relatively severe damage. The biochemistry index and histomorphology observation indicates that adenine CRF rat modeling was successful, the specific harm and acidosis of renal cortex and renal medulla function of model group B conformed to the common biochemistry target changes of chronic kidney function failure in the mid and late stage of urenfia. Conclusion： The method with gastric perfusion of 2. 5% adenine suspensions （250 mg/kg/d） for 14 days and then every, other day given medicine for anotherl4 days is much better.