| 抗人绒毛膜促性腺激素单克隆抗体的制备与鉴定 |
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| 引用本文： | 李伟雄,孙敏之,马守兴,王海云,徐一树. 抗人绒毛膜促性腺激素单克隆抗体的制备与鉴定[J]. 生殖与避孕, 1986, 0(3) |
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| 作者姓名： | 李伟雄 孙敏之 马守兴 王海云 徐一树 |
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| 作者单位： | 北京医学院基础医学研究所(李伟雄，孙敏之，马守兴)，中国科学院动物研究所内分泌室(王海云)，中国科学院动物研究所内分泌室(徐一树) |
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| 基金项目： | 世界卫生组织人类生殖特别规划处的资助。 |
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| 摘 要： | 利用杂交瘤技术制备了11株抗人绒毛膜促性腺激素(hCG)的单克隆抗体。根据这些抗体和~(125)I 标记的hCG,hCGα,hCGβ和hLH 结合的情况,可将抗体分为三类。抗体BAH_2作用于hCG_α和β亚单位结合后所暴露出的抗原决定簇,抗体10E_5和8D_5特异地和hCGβ亚单位结合但不和hLH 结合。大多数抗体是作用于hCG 和hLHβ亚单位上共有的决定簇。实验显示这些单克隆抗体特异性优于多克隆抗体,它们亲合常数在5.3×10_7～7.2×10~(10)M~(-1)之间。
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PREPARATION AND CHARACTERIZATION OF ANTI-hCG MONOCLONAL ANTIBODIES |
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| Li Wei-xiong Sun Min-zhi Ma Shou-xing. PREPARATION AND CHARACTERIZATION OF ANTI-hCG MONOCLONAL ANTIBODIES[J]. Reproduction and Contraception, 1986, 0(3) |
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| Authors: | Li Wei-xiong Sun Min-zhi Ma Shou-xing |
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| Abstract: | Eleven strains of anti-hCG monoclonal antibodies were prepared.These antibodieswere tested for binding with ~(125)I labelled hCG,hCGα,hCGβ and hLH.Antibody BAH_2acted against an epitope which was exposed only after α and β subunits combination.Most ofthe antibodies could bind with hCG,hCGβ or hLH.This suggests that these antibodieswere against the common epitopes of hCGβand hLHβ.Antibodies 10E_5 and 8D_5 only boundwith the specific epitopes of hCGβ.The affinity constants of these antibodies were between5.3×10~7M~(-1)～7.2×10~(10)M~(-1): |
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| Keywords: | Anti-hCG Monoclonal antibody Hybridoma |
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