肾综合征出血热患者外周血循环内皮细胞数量变化及HSP70的表达

目的探讨肾综合征出血热(HFRS)患者外周血循环内皮细胞(CEC)的数量变化及热休克蛋白70（HSP70）表达的意义。方法54例HFRS患者按病情分为轻型20例，中型20例，重型及危重型14例，另取20例健康者作为对照。每例均按病期采血，采用Percoll密度梯度离心法分离内皮细胞后，应用流式细胞技术检测CEC数量及CEC中HSP70的表达；分析不同病期、不同临床类型HFRS患者CEC、HSP70变化的规律。结果在不同病期的HFRS患者中，CEC计数及HSP70的表达在发热期已升高，在低血压期和少尿期持续高水平，至多尿期及恢复期逐渐降低；在不同临床类型的HFRS患者中，随病情加重，CEC数量及HSP70的表达亦相应增高，在重型和危重型患者中达到高峰。与健康对照组相比，不同病期、不同临床类型的HFRS患者CEC数量及HSP70表达差异有统计学意义(P＜0.05)。结论HFRS患者血CEC数量和CEC中HSP70表达均增高，其增高的程度与病期和临床类型有关。

Changes of circulation endothelial cells and HSP70 in hemorrhagic fever with renal syndrome

Objective To explore the significance of changes of circulation endothelial cells(CECs)and of the expression of heat-shock protein70(HSP70) in patients with hemorrhagic fever with renal syndrome(HFRS).Method Fifty-four HFRS patients were assigned to the mild group(20 cases),the moderate group(20 cases) and the severe group(14 cases),and 20 healthy volunteers served as the controls.After CECs were separated by using the isopyknic centrifugation method,the CEC number and HSP70 level were determined with flow cytometry.Results The number of CEC and the expression of HSP70 of HFRS patients began to increase in the fever stage and maintained a high level in the hypotension and oliguria stages,then gradually decreased in the diuretic phase and the recovery phase.In patients with different clinical types of HFRS,the CEC number and the HSP70 level accordingly increased and reached the peak in severe and dangerous HFRS.In patients with different stages and types of HFRS,the number of CEC and expression of HSP70 were both significantly higher than those of the controls.Conclusion The CEC number and expression of HSP70 both increase in HFRS patients and this is related to the stage and clinical type of HFRS.